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Addgene

We narrowed to 11,463 results for: 110

Showing: 11081 - 11100 of 11463 results
  1. LLP185 pLVP-dCas9-DNMT3a V2

    Plasmid
    #100936
    Purpose
    dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone
    Depositor
    Insert
    dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and 3xTy1
    Expression
    Mammalian
    Mutation
    D10A, H840A for dCas9
    Promoter
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA3.1+CMV-Ins-GLuc

    Plasmid
    #89929
    Purpose
    Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Insert
    human insulin-Gaussia-Luciferase (INS Human, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    CMV
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. Ad:2CMV_A2R2-N25Ctr

    Plasmid
    #122243
    Purpose
    Expresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone
    Depositor
    Inserts
    C-terminal end of Nesprin-3 w/o Kash (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    Use
    Adenoviral
    Tags
    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…
    Expression
    Mammalian
    Mutation
    deleted Kash domain (GGCGAGGAGGAGCGCAGCTGCGCCCTGG…
    Promoter
    CMV
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. TFORF1959

    Plasmid
    #143986
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Insert
    CUX1 (CUX1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pAAV-STAT-CA

    Plasmid
    #195576
    Purpose
    Expresses STAT3 constitutively active mutant
    Depositor
    Insert
    Signal transducer and activator of transcription 3 (Stat3 Mouse)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    changed Alanine 662 to Cysteine and Asparagine 66…
    Promoter
    CMV enhancer and promoter
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pαH-S-GSAS-2P-Omicron-BA.5

    Plasmid
    #213828
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.2P.BA5 variant
    Depositor
    Insert
    pαH-S-GSAS/Omicron-2P-BA5 (S )
    Use
    Tags
    HRV 3C cleavage site (before tags) 8X His tag 2X…
    Expression
    Mammalian
    Mutation
    G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…
    Promoter
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 11081 - 11100 of 11463 results