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pLenti-mEtfdh-AAA4

Plasmid

#232316

Purpose

Expression of mouse mutant Etfdh (AAA4)

Depositor

Ivan Topisirovic

Article

Papadopoli et al bioRxiv 2024.10.25.620155

Insert

Etfdh (Etfdh Mouse)

Use

Lentiviral

Mutation

PAM Sequence Mutations: (G36C; T39C, C42T; C72T, …

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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MKC89_CCR5(UbC-tEPOR-2A-YFP)

Plasmid

#232404

Purpose

AAV production plasmid for UbC-tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at CCR5 locus using CCR5-sg3 gRNA. YFP is followed by a BGH polyA. AAV genome is flanked by AAV2 ITRs.

Depositor

Kyle Cromer, Matthew Porteus

Article

Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552

Insert

Truncated Erythropoietin Receptor (EPOR Human)

Use

AAV

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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MKC91_tEPOR(UbC-GFP-BGH)

Plasmid

#232406

Purpose

AAV production plasmid for tEPOR-UbC-GFP-BGH vector from Fig. 1 that mediates HDR at EPOR locus using EPOR-sg1 gRNA. Repair vector introduces premature stop codon into EPOR locus.

Depositor

Kyle Cromer, Matthew Porteus

Article

Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552

Insert

Truncated Erythropoietin Receptor (EPOR Human)

Use

AAV

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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rag2:CPSF6_CE_pISceI

Plasmid

#223037

Purpose

Express CPSF6 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

CPSF6 (CPSF6 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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IL2-AHDC2-Cherry in pmCherryN1

Plasmid

#187291

Purpose

Express pmCherry-IL2-AH-deltaC2

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Scraps interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)

Tags

mCherry

Expression

Mammalian

Mutation

AH-deltaC2

Promoter

CMV

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-MYC(Δ1-143)-GFP-IRES-mCherry (ΔTAD)

Plasmid

#231233

Purpose

MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) for transient expression in mammalian cells

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

MYC (MYC Human)

Expression

Mammalian

Mutation

TAD deleted (delta1-143); numbering based on sequ…

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-HA-MycΔdeg1∆deg2

Plasmid

#231243

Purpose

HA-tagged MYC over-expession construct with point mutations corresponding to the deletion of degron 1 and 2 for transient expression in mammalian cells

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

MYC (MYC Human)

Expression

Mammalian

Mutation

Deletion of MYC degrons 1 & 2 (K355E; R356E; …

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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CRaf-IRES-pErk reporter

Plasmid

#226170

Purpose

Used to transiently express Myc-tagged CRaf with the pErk reporter. Used in LABEL-seq MAPK activity assays.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

Myc-CRaf-IRES-3xFlag-tdMCP-Erk2 (MAPK1, RAF1 Human)

Tags

3xFlag-tdMCP and Myc

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMV10 lenti pEF-rTetR(SE-G72P)-3XFLAG-WWP2(WW)-T2A-mCherry-BSD-WPRE

Plasmid

#188756

Purpose

Lentiviral backbone containing WWP2 WW domain as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

WWP2 WW (WWP2 Human)

Use

Lentiviral

Tags

rTetR(SE-G72P)-3xFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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SHIP2-ΔPR1

Plasmid

#214902

Purpose

expression of the truncated variants of the SHIP2 without first proline-rich domain

Depositor

Pavel Krejčí

Article

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 delta aa 123-410 (INPPL1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

delta 123-410aa

Promoter

CMV

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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SHIP2-C2

Plasmid

#214906

Purpose

expression of the truncated variants of the SHIP2 that retains second proline-rich domain

Depositor

Pavel Krejčí

Article

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 aa 740-1189 (INPPL1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

delta 1-739, 1190-1258aa

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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SHIP2-C1

Plasmid

#214905

Purpose

expression of the truncated variants of the SHIP2 that retains second proline-rich domain and C-terminal sterile alpha motif

Depositor

Pavel Krejčí

Article

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 aa 740-1258 (INPPL1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

delta 1-739aa

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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SHIP2-N2

Plasmid

#214904

Purpose

expression of the truncated variants of the SHIP2 that retains first proline-rich domain

Depositor

Pavel Krejčí

Article

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 aa 123-418 (INPPL1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

delta 1-122, 419-1258aa

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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SHIP2-N1

Plasmid

#214903

Purpose

expression of the truncated variants of the SHIP2 that retains only SH2 domain and first proline-rich domain

Depositor

Pavel Krejčí

Article

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 aa 1-418 (INPPL1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

delta 419-1258aa

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-VMAT1 [N440/61R]

Plasmid

#206580

Purpose

Mammalian Expression Plasmid of anti-VMAT1 (Mouse). Derived from hybridoma N440/61.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VMAT1 (Mus musculus) recombinant Mouse monoclonal antibody (Slc18a1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-VMAT1 [N440/21R]

Plasmid

#206579

Purpose

Mammalian Expression Plasmid of anti-VMAT1 (Mouse). Derived from hybridoma N440/21.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VMAT1 (Mus musculus) recombinant Mouse monoclonal antibody (Slc18a1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_TRIOBP_RBFOX2

Plasmid

#205947

Purpose

Express mEGFP-tagged fusion protein, TRIOBP_RBFOX2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

TRIOBP_RBFOX2_L (TRIOBP, RBFOX2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ITGB6_RBMS1

Plasmid

#205831

Purpose

Express mEGFP-tagged fusion protein, ITGB6_RBMS1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ITGB6_RBMS1 (ITGB6, RBMS1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-hSOX2-C17

Plasmid

#206385

Purpose

Expresses human SOX2-C17 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

SOX2-C17 chimera (SOX2, SOX17 Human)

Use

Lentiviral

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-LRIG2-COMP5AP-AviTag-9xHis

Plasmid

#157540

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LRIG2 (LRIG2 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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