We narrowed to 4,150 results for: puc19

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pUC19-U6-MbCas12a crRNA-BsmBI cassette (BPK4449)

Plasmid

#114088

Purpose

U6 promoter crRNA entry vector used for all MbCas12a crRNAs (clone spacer oligos into BsmBI cassette)

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for MbCas12a crRNAs

Expression

Mammalian

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTR-341 pUC19-tNGFR-P2A-PDCD1 N

Plasmid

#186074

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

PDCD1 (PDCD1 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-348 pUC19-tNGFR-P2A-ICOS N

Plasmid

#186066

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

ICOS (ICOS Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-337 pUC19-tNGFR-P2A-CTLA4 N

Plasmid

#186054

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CTLA4 (CTLA4 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-342 pUC19-tNGFR-P2A-CD28 N

Plasmid

#186056

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CD28 (CD28 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19 KGA HA5'-mKO2-P2A-Zeo-3'

Plasmid

#110408

Purpose

Homology donor for glutaminase KGA isoform knock-in, mKO2-P2A-Zeo

Depositor

Sandra Martha Gomes Dias

Insert

GLS glutaminase (GLS Human)

Use

CRISPR

Promoter

none

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)

Plasmid

#154824

Purpose

(eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegans

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

Peft-3::wrmScarlet::tbb-2 UTR

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

Plasmid

#215769

Purpose

encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus

Depositor

Dimitrios Wagner

Article

Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:

Inserts

TRAC-5' homology arm (TRAC Human)
P2A-CD19.28.zeta-bgH (CD19 Mouse, Human, Bovine)
TRAC-3' homology arm (TRAC Human)

Expression

Bacterial

Mutation

Cas12a PAM mutated

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

Plasmid

#174025

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

eGFP

Use

Zebrafish knock-in tagging

Mutation

Y40N

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pTR-345 pUC19-tNGFR-P2A-IL2RB N

Plasmid

#186064

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

IL2RB (IL2RB Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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