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Plasmid#86698PurposeCas9 expression vector for targeting to the AAVS1 locusDepositorInsertCas9
UseCRISPRTagsExpressionMammalianMutationPromoterpCagAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
iSH-iRFP670-sspB
Plasmid#176102PurposeHeterodimerization with iLID, phosphatidylinositol 3-kinase derived iSHDepositorInsertphosphatidylinositol 3-kinase (Pik3r1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
AA435
Plasmid#215962PurposeFragmid fragment: (guide cassette) guide expression; positive control cell surfaceDepositorHas ServiceCloning Grade DNAInsertU6_v1; DR_v0 [EnAs]; sgCD274_v1; DR_v1 [EnAs]; sgADGRE5_v3; DR_v2; sgCD4_v1; DR_v3; sgDPP4_v1
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
SQ171/pCSacB/ptRNA100
Bacterial Strain#155206PurposeSQ171 strain supported by pCSacB and ptRNA100 plasmidsDepositorBacterial ResistanceKanamycinAvailable sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PHGDH
Plasmid#74113PurposeExpression of N-terminal His-taggedDepositorInsertPHGDH (PHGDH Human)
UseTagsHis-taggedExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
SSpB-HaloTag-p63DH
Plasmid#176116PurposeHeterodimerization with iLID, RhoA activationDepositorInsertRHOGEF p63 (ARHGEF25 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
SSpB-mCherry-p63DH
Plasmid#176112PurposeHeterodimerization with iLID, RhoA activationDepositorInsertRHOGEF p63 (ARHGEF25 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
SSpB-iRFP-p63DH
Plasmid#176120PurposeHeterodimerization with iLID, RhoA activationDepositorInsertRHOGEF p63 (ARHGEF25 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-SspB-HaloTag-p63DH
Plasmid#176129PurposeHeterodimerization with iLID, RhoA activationDepositorInsertRHOGEF p63 (ARHGEF25 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF3119
Plasmid#144595PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertDDIT3 (DDIT3 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-SspB-HaloTag-ITSN1-DHPH
Plasmid#176128PurposeHeterodimerization with iLID, Cdc42 activationDepositorInsertITSN1 (ITSN1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBOBI C-FLAG PHGDH
Plasmid#212995Purposeexpresses human PHGDHDepositorInsertPHGDH (PHGDH Human)
UseTagsFLAGExpressionMammalianMutationPromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_YY1_WT
Plasmid#82171PurposeGateway Donor vector containing YY1 , part of the Target Accelerator Plasmid Collection.DepositorInsertYY1 (YY1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF0623
Plasmid#142666PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertDDIT3 (DDIT3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
SspB-HaloTag-DHPH-ITSN1
Plasmid#176113PurposeHeterodimerization with iLID, Cdc42 activationDepositorInsertITSN1 (ITSN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
SspB-iRFP-DHPH-ITSN1
Plasmid#176117PurposeHeterodimerization with iLID, Cdc42 activationDepositorInsertITSN1 (ITSN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
LMPd Amt PHGDH
Plasmid#209409PurposeRetroviral vector with Ametrine marker for expressing shRNA with an "UltramiR" microRNA scaffoldDepositorInsertPhgdh shRNA (Phgdh Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFRSF13B-COMP5AP-AviTag-9xHis
Plasmid#157126PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertTNFRSF13B (TNFRSF13B Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
SspB-mCherry-DHPH-ITSN1
Plasmid#176103PurposeHeterodimerization with iLID, Cdc42 activationDepositorInsertITSN1 (ITSN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM5-WT PHGDH
Plasmid#83901Purposestable overexpressionDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-PHGDH
Plasmid#83907Purposestable overexpressionDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_DDIT3_WT
Plasmid#82107PurposeGateway Donor vector containing DDIT3 , part of the Target Accelerator Plasmid Collection.DepositorInsertDDIT3 (DDIT3 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-PHGDH-C234S
Plasmid#107724PurposeExpression of PHGDH C234S in bacteriaDepositorInsertPHGDH (PHGDH Human)
UseTagsExpressionBacterialMutationC234SPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1-sgPHGDH-G5
Plasmid#83913Purposestable knockoutDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM5-C234S PHGDH
Plasmid#83902Purposestable overexpressionDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseLentiviralTagsExpressionMammalianMutationquikchanged cysteine 234 to serinePromoterCMVAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBOBI C-FLAG PHGDH V261M
Plasmid#212999Purposeexpresses human PHGDHDepositorInsertPHGDH (PHGDH Human)
UseTagsFLAGExpressionMammalianMutationV261MPromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFRSF13B-Fc(DAPA)-AviTag-6xHis
Plasmid#156561PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertTNFRSF13B (TNFRSF13B Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBOBI C-FLAG PHGDH T78A
Plasmid#212997Purposeexpresses human PHGDHDepositorInsertPHGDH (PHGDH Human)
UseTagsFLAGExpressionMammalianMutationT78APromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBOBI C-FLAG PHGDH R135W
Plasmid#212998Purposeexpresses human PHGDHDepositorInsertPHGDH (PHGDH Human)
UseTagsFLAGExpressionMammalianMutationR135WPromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBOBI C-FLAG PHGDH T57A
Plasmid#212996Purposeexpresses human PHGDHDepositorInsertPHGDH (PHGDH Human)
UseTagsFLAGExpressionMammalianMutationT57APromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only