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We narrowed to 32,009 results for: Eng

Showing: 1101 - 1120 of 32009 results

  1. pCHC030 (∆CBBp ∆MBH ∆SH)

    Plasmid
    #226205
    Purpose
    Electroporation knockout plasmid (pK18sB) to delete the CBBp, membrane-bound hydrogenase, soluble hydrogenase, and intervening sequences (∆CBBp ∆MBH ∆SH).
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Homology arms for CBBp, MBH, and SH operons, and intervening sequencessequences.
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ∆cbbR ∆cbbLSXYEFPTZGKA ∆hoxKGZMLOQRTV ∆hypA1B1F1C…
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226205-plasmid-map-sequence-id-453511

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  2. pCHC036 (∆pHG1)

    Plasmid
    #226206
    Purpose
    Conjugation knockout plasmid (pK18msB) to delete the C. necator megaplasmid replication region containing helD, repA, repB, and parAB in order to promote loss of the entire pHG1 megaplasmid.
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Homology arms for the pHG1 megaplasmid replication region
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ∆helD ∆repA ∆repB ∆parAB
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226206-plasmid-map-sequence-id-452437

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  3. pACB120

    Plasmid
    #222213
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with five mutations (K10N, S11R, W15C, G76V, G258D) and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(K10N, S11R, W15C, G76V, G258D)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Contains five amino acid mutations: K10N, S11R, W…
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222213-plasmid-map-sequence-id-443111

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  4. pACB143

    Plasmid
    #222214
    Purpose
    For recombinant expression of vanB (PP_3737 from Pseudomonas putida) with the A24P mutation and an N-terminal thrombin-cleavable His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    vanB(A24P)
    Use
    Tags
    N-terminal, thrombin-cleavable 6x His tag
    Expression
    Bacterial
    Mutation
    Changed Alanine 24 to Proline
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222214-plasmid-map-sequence-id-438176

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  6. pACB111

    Plasmid
    #222210
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G76V mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(G76V)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed glycine 76 to valine
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222210-plasmid-map-sequence-id-437376

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  7. pACB112

    Plasmid
    #222211
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the S11R mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(S11R)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Serine 11 to Arginine
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222211-plasmid-map-sequence-id-437378

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  8. pACB113

    Plasmid
    #222212
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(W15C)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Tryptophan 15 to Cysteine
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222212-plasmid-map-sequence-id-437381

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  18. Coexpressed Free NES

    Plasmid
    #182482
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS
    NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10 and GAL7
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182482-plasmid-map-sequence-id-360445

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  19. Coexpressed Free PcPTS

    Plasmid
    #182483
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and patchoulol synthase from Pogostemon cablin (PcPTS; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS
    PTS
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10 and GAL7
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182483-plasmid-map-sequence-id-360444

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  20. Linked Free NES (AG4TGGA)2

    Plasmid
    #182488
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence (AG4TGGA)2 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Insert
    FPPS-NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182488-plasmid-map-sequence-id-360434

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Showing: 1101 - 1120 of 32009 results