We narrowed to 5,507 results for: SPI

1. pVRC8400-SARS-CoV-2-RBD-BA.4/5-AVI

   Plasmid

   #192114

   Purpose

   Expresses SARS-CoV-2 RBD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Insert

   SARS-CoV-2-RBD-BA.4/5 (S )
   

   Use

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   G339D, S371F, S373P, S375F, T376A, D405N, R408S, …

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMSCV-Flag-Zfp423-deltaSBD

   Plasmid

   #35971

   Depositor

   Bruce Spiegelman

   Article

   Gupta et al Nature. 2010 Mar 3. ():.

   Insert

   Zinc Finger Protein 423 (Zfp423 Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   lacks zinc fingers 14-19 (aa 640 -838) (delta SMA…

   Promoter

   Available since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pCMX-PGC1

   Plasmid

   #6

   Depositor

   Bruce Spiegelman

   Article

   Puigserver et al Cell 1998 Mar 20;92(6):829-39.

   Insert

   PGC-1 alpha (Ppargc1a Mouse)
   

   Use

   Tags

   Gal4

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pENTR4_EWSR1

   Plasmid

   #26367

   Depositor

   Thomas Tuschl

   Article

   Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pB-DEST (JDW 1205)

   Plasmid

   #229821

   Purpose

   A PiggyBac destination vector compatible with gateway cloning system.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pB-Neo-DEST (JDW 940)

   Plasmid

   #229825

   Purpose

   A neo selectable, PiggyBac destination vector compatible with gateway cloning system.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2-phiC31o (JDW 453)

   Plasmid

   #229842

   Purpose

   A CMV driven Phi31 recombinase.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   phiC31 codon optimized, PhiC31o
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p3E-V5-mTagBFP2 (JDW 871)

   Plasmid

   #224531

   Purpose

   A Gateway compatible 3' entry clone containing a V5 tagged mTagBFP2

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   V5-mTagBFP2
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LCB1_v1.3 (MTK 3a) (pZYW044)

   Plasmid

   #194227

   Purpose

   Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB1

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   LCB1_v1.3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LCB3_v1.2 (MTK 3a) (pZYW045)

    Plasmid

    #194228

    Purpose

    Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB3

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    LCB3_v1.2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA4/TO-Cdc45HA

    Plasmid

    #211427

    Purpose

    tet-inducible mammalian expression of human Cdc45 C-terminally fused to HA-Tag

    Depositor

    Helmut Pospiech

    Article

    Kohler et al Cell Cycle. 2016;15(7):974-85. doi: 10.1

    Insert

    Cdc45 (CDC45 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL4.11_Nued2_E1_dAP1-2

    Plasmid

    #203837

    Purpose

    Luciferase reporter plasmid for Igf1 enhancer 1 with both AP-1 sites knocked out

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    dAP1-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    KO of both AP1 sites

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL4.11_Nued2_E1_dAP1

    Plasmid

    #203835

    Purpose

    Luciferase reporter plasmid for Igf1 enhancer 1 containing only the downstream AP1 site

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    dAP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    KO of AP1 upstream

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL4.11_Nued2_E1_dAP2

    Plasmid

    #203836

    Purpose

    Luciferase reporter plasmid for Igf1 enhancer 1 containing only the upstream AP1 site

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    dAP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    KO of AP1 downstream

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCZGY921

    Plasmid

    #135092

    Purpose

    Transcriptional GFP reporter of acr-2 (cholinergic) expressing neurons in C. elegans

    Depositor

    Yishi Jin

    Article

    Jospin et al PLoS Biol. 2009 Dec;7(12):e1000265. doi:

    Insert

    Pacr-2
    

    Use

    Tags

    GFP and Synthetic Intron

    Expression

    Worm

    Mutation

    Promoter

    Pacr-2

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGEN-WPRE-bGH-DEST (JDW 1309)

    Plasmid

    #229815

    Purpose

    A CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcripts

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pME-3x-FLAG-Ubiquitin (JDW 1098)

    Plasmid

    #224513

    Purpose

    A Gateway compatible middle entry clone containing a 3x FLAG tagged human Ubiquitin for monitoring ubiquitylation status

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    3x-FLAG-Ubiquitin
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-Ef1a-fDIO-mRuby3-2A-Cre

    Plasmid

    #203844

    Purpose

    Flp-dependent Cre and mRuby3 expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    Cre
    

    Use

    AAV

    Tags

    mRuby3-2A

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCV-BLU-VIPR

    Plasmid

    #220497

    Purpose

    Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).

    Depositor

    Alexander Espinosa

    Article

    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBLU-VIPR

    Plasmid

    #220498

    Purpose

    Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).

    Depositor

    Alexander Espinosa

    Article

    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

    Type

    Empty backbone

    Use

    CRISPR; Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge