We narrowed to 15,703 results for: gRNA

1. pAcHELIX-sgRNA_entry (CJT94)

   Plasmid

   #181783

   Purpose

   Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BRCA2 A11.2 gRNA

   Plasmid

   #90550

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BRCA2

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   BRCA2 (Guide Designation A11.2)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSB-BbsI-sgRNA

   Plasmid

   #203359

   Purpose

   Contains BbsI cut sites for placement of user-specified sgRNA target sequences with Sleeping Beauty backbone

   Depositor

   Jonathan Brunger

   Article

   Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

   Insert

   BbsI Cut Sites
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSCAR_sgRNA_hygro-tagBFP-lox5171

   Plasmid

   #162070

   Purpose

   3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX458_mCherry-SRRM2-gRNA

   Plasmid

   #238251

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to SRRM2 locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   SRRM2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Pepper-fused sgRNA

   Plasmid

   #217520

   Purpose

   Expresses Pepper-fused sgRNA in mammalian cells with U6 promoter

   Depositor

   Xing Li

   Article

   Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

   Insert

   Pepper-fused sgRNA for spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W

   Plasmid

   #163169

   Purpose

   Lentiviral plasmid with non-targeting gRNA, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   Non-targeting
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-P3-evoCjCas9-ABE8e_gRNA

   Plasmid

   #202559

   Purpose

   P3-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-ABE8e
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pU6 SgRNA RBM20

   Plasmid

   #162735

   Purpose

   Mammalian expression

   Depositor

   Amit Choudhary

   Article

   Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

   Insert

   Spacer sequence for RBM20
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DYRK1A gRNA (BRDN0001145031)

    Plasmid

    #76755

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DYRK1A

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DYRK1A (DYRK1A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-BFP-U6-sgRNA

    Plasmid

    #196725

    Purpose

    Sortable sgRNA expression, Chen tracrRNA PMID: 24360272

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. gRNA-MS2x2-TagRFP657

    Plasmid

    #184441

    Purpose

    sgRNA with MS2 aptamer, TagRFP657 as selection marker

    Depositor

    Rhys Allan

    Article

    Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

    Insert

    sgRNA-MS2x2, TagRFP657
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p(U6a-BsaI-gRNA)

    Plasmid

    #65955

    Purpose

    Tribolium U6a promoter with BsaI cloning site to insert guide RNA sequence

    Depositor

    Michalis Averof

    Article

    Gilles et al Development. 2015 Jul 9. pii: dev.125054

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSIN-Arkadia-gRNA1

    Plasmid

    #180374

    Purpose

    targeting mouse Arkadia gene

    Depositor

    Dan Littman

    Article

    Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

    Insert

    Arkadia targeting gRNA (Rnf111 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-memRFP-3xControlgRNA

    Plasmid

    #224567

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    mRFP1
    

    Use

    CRISPR

    Tags

    Membrane localization signal

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PKD1 gRNA (BRDN0001148399)

    Plasmid

    #76842

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKD1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKD1 (PKD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PKD1 gRNA (BRDN0001149544)

    Plasmid

    #76841

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKD1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKD1 (PKD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Non-specific sgRNA

    Plasmid

    #109432

    Purpose

    MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Non-specific gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLQ1651-sgRNA(F+E)-sgGal4

    Plasmid

    #100549

    Purpose

    sgGal4-expressing vector modified from Addgene plasmid #51024

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Insert

    sgGal4
    

    Use

    CRISPR

    Tags

    sgRNA

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RNASEL gRNA (BRDN0001149212)

    Plasmid

    #77051

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge