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Showing: 1101 - 1120 of 12704 results

  3. PtPuc3_diaCas9_sgRNA

    Plasmid
    #109219
    Purpose
    Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum
    Depositor
    Per Winge
    Article
    Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10
    Inserts
    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    LHCF1 terminator, LHCF2 promoter, U6 3' regi…
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    109219-plasmid-map-sequence-id-260727

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  9. pET28a-baPrs-hdNadV

    Plasmid
    #91950
    Purpose
    pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi
    Depositor
    George Cătălin Marinescu
    Article
    Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10
    Inserts
    Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
    Phosphoribosyl Pyrophosphate Synthetases
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed Leucine 135 to Isoleucine
    Promoter
    T7
    Available since
    Sept. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    91950-plasmid-map-sequence-id-194125

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  10. pEVA1

    Plasmid
    #58953
    Purpose
    Contains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.
    Depositor
    Thomas Mason, Thomas Fox
    Article
    Sanchirico et al EMBO J. 1998 Oct 1;17(19):5796-804.
    Insert
    VAR1u, VAR1 recoded for expression from the nucleus
    Use
    Yeast ecoli shuttle vector
    Tags
    preCox4 mitochondrial matrix targeting signal
    Expression
    Yeast
    Mutation
    Synthetic gene in standard genetic code
    Promoter
    ADH1
    Available since
    Aug. 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pcDNA3.1-CHRFAM7ADelta2bp-mGFP

    Plasmid
    #62636
    Purpose
    mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mEGFP fused into M3-M4 loop
    Depositor
    Henry Lester
    Article
    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6
    Insert
    CHRFAM7A (CHRFAM7A Human)
    Use
    Tags
    mEGFP inserted in M3-M4 loop
    Expression
    Mammalian
    Mutation
    there is a 2-bp deletion in exon 6 of CHRFAM7A ca…
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pJOG252

    Plasmid
    #80584
    Purpose
    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII
    Depositor
    Johannes Stuttmann
    Article
    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1
    Inserts
    pnos:nptII-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A
    Promoter
    Available since
    Sept. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    80584-plasmid-map-sequence-id-151870

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  15. pJOG253

    Plasmid
    #80585
    Purpose
    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA
    Depositor
    Johannes Stuttmann
    Article
    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1
    Inserts
    pnos:PAT-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A
    Promoter
    Available since
    Sept. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    80585-plasmid-map-sequence-id-151872

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Showing: 1101 - 1120 of 12704 results