We narrowed to 6,650 results for: tac

1. FKBP-EGFP-RanGAP*

   Plasmid

   #175238

   Purpose

   Bacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the Km

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   RANGAP1 (RANGAP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Amino acid 562 F to A

   Promoter

   tac

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX-PP-NEDD4L-WW1(193-226)

   Plasmid

   #166701

   Purpose

   expresses NEDD4L WW1 in bacterial cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   NEDD4L WW1 domain
   

   Use

   Tags

   GST, HRV 3C site, and Thrombin site

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-PP-NEDD4L-WW2(385-418)

   Plasmid

   #166702

   Purpose

   expresses NEDD4L WW2 in bacterial cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   NEDD4L WW2 domain
   

   Use

   Tags

   GST, HRV 3C site, and Thrombin site

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX-PP-NEDD4L-WW3(497-530)

   Plasmid

   #166703

   Purpose

   expresses NEDD4L WW3 in bacterial cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   NEDD4L WW3 domain
   

   Use

   Tags

   GST, HRV 3C site, and Thrombin site

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-PP-NEDD4L-WW4(548-581)

   Plasmid

   #166704

   Purpose

   expresses NEDD4L WW4 in bacterial cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   NEDD4L WW4 domain
   

   Use

   Tags

   GST, HRV 3C site, and Thrombin site

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EcNGsC-MutY-pKK223

   Plasmid

   #131376

   Purpose

   Low level expression of E.coli G.stearothermophillus MutY chimera protein

   Depositor

   Martin Horvath

   Article

   Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

   Insert

   EcNGsC MutY chimera
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Fusion of two MutY genes. Residues 1-225 are from…

   Promoter

   tac

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-4T-3-cytXlATL2-R232Q

   Plasmid

   #124064

   Purpose

   Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

   Depositor

   Tom Rapoport

   Article

   Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

   Insert

   cytXlATL2-R232Q
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFM101

   Plasmid

   #40147

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   gfp::par-2 wild-type RNAi resistant (par-2 Nematode)
   

   Use

   Tags

   GST and eGFP

   Expression

   Bacterial

   Mutation

   Base pairs 6-523 of par-2 were recoded to create …

   Promoter

   tac

   Available since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pFM102

   Plasmid

   #40148

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   gfp::par-2 RNAi resistant R183-5A (par-2 Nematode)
   

   Use

   Tags

   GST and eGFP

   Expression

   Bacterial

   Mutation

   R183-5A; base pairs 6-523 of par-2 were recoded t…

   Promoter

   tac

   Available since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pJK01_Rho_minprox_DsREd

    Plasmid

    #173489

    Purpose

    PCR template for reporter gene with mouse Rhodopsin promoter with DsRed

    Depositor

    Barak Cohen

    Article

    White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

    Insert

    Mouse Rhodopsin promoter driving DsRed reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Rhodopsin

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Musunuru-Cowan TALEN Kit

    Plasmid Kit

    #1000000034

    Purpose

    Used to efficiently assemble TALEN constructs with custom repeat arrays, each containing 15 repeats. Contains pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly.

    Depositor

    Kiran Musunuru

    Available since

    July 19, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pSLQ7535 pHR (hU6-crSURF2-EFS-PuroR-WPRE)

    Plasmid

    #214879

    Purpose

    Lentiviral vector encoding RfxCas13d targeting SURF2 guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crSURF2-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAM/CBA-eGFP-miRNegativex3-WPRE-bGHpA

    Plasmid

    #194249

    Purpose

    Expresses 3 non-targeting miRNAs

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    miRNegative
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX4T3-GST-NQO1

    Plasmid

    #177140

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of NQO1 with a TEV protease site located between the GST tag and NQO1

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    NQO1 (NQO1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRARE-MBP-DEST

    Plasmid

    #84650

    Purpose

    Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

    Depositor

    Neil Olszewski

    Article

    Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

    Type

    Empty backbone

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-6P-1_WT RAD23B

    Plasmid

    #201457

    Purpose

    Expresses human RAD23B with an N-terminal GST tag in E. coli

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Coding sequence has been optimised for expression…

    Promoter

    tac promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PDLIM4_PDZ_1

    Plasmid

    #103941

    Purpose

    Protein expression and purification of RIL BGLII PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDLIM4 (PDLIM4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-6P-1 SARS-CoV 3CLpro

    Plasmid

    #61692

    Purpose

    inducible expression of SARS 3CLpro protease

    Depositor

    Michael Johnson

    Article

    Lee et al Bioorg Med Chem. 2014 Jan 1;22(1):167-77

    Insert

    SARS 3CLpro protease
    

    Use

    Tags

    GST and HIS

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. SNRPA_Full-length_pGEX

    Plasmid

    #135134

    Purpose

    Recombinant expression of SNRPA with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    SNRPA (SNRPA Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Feb. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUCXMG

    Plasmid

    #204409

    Purpose

    Expression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI site

    Depositor

    David Ackerley

    Article

    Rich et al bioRxiv. 2023 Mar 25:2023.03.24.534183.

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge