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pLigify_Isoeugenol

Plasmid

#215563

Purpose

Expresses GFP in response to Isoeugenol

Depositor

Simon d'Oelsnitz, David Ross

Article

d'Oelsnitz et al ACS Synth Biol. 2024 Aug 16;13(8):2577-2

Inserts

GFP mut2
iemR

Expression

Bacterial

Promoter

P250 and Piemr

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLigify_4Vinylphenol

Plasmid

#215564

Purpose

Expresses GFP in response to 4-Vinylphenol

Depositor

Simon d'Oelsnitz, David Ross

Article

d'Oelsnitz et al ACS Synth Biol. 2024 Aug 16;13(8):2577-2

Inserts

GFP mut2
vprR

Expression

Bacterial

Promoter

Pvprr

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDonorTp-fugfp

Plasmid

#211790

Purpose

pDonorTp with addition of fugfp gene from pUS252 outside of the FRT sites

Depositor

Silvia Cardona

Article

Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

Insert

fuGFP

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p500-Erg6-V5-APEX2-URA

Plasmid

#207070

Purpose

Expressing p500-Erg6-V5-APEX2. Stains cell peripheral in addition to lipid droplets.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Erg6

Tags

V5-APEX2

Expression

Yeast

Promoter

pERG6

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-OM14-V5-APEX2-Ura

Plasmid

#207073

Purpose

Expressing OM14-V5-APEX2. Marker for mitochondrial outer membrane.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

OM14

Tags

V5-APEX2

Expression

Yeast

Promoter

pOM14

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-3C-hTspan12LEL(118-218)_pAcGP67a

Plasmid

#216383

Purpose

Baculovirus transfer vector to secrete human Tspan12 LEL (residues 118-218) fused to MBP

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Tetraspanin-12 (TSPAN12 Human)

Use

Baculovirus

Tags

GP64 signal sequence-MBP-3C

Expression

Insect

Promoter

Polyhedrin

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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Pxyl-rsaA

Plasmid

#207250

Purpose

Integrative plasmid for expressing rsaA gene (incl. its promoter) in Caulobacter crescentus at native Pxyl promoter region

Depositor

Suliana Manley

Article

Zhang et al Nat Commun. 2024 Apr 24;15(1):3460. doi:

Insert

rsaA (promoter to codon sequence)

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB602-po-mCerulean

Plasmid

#213861

Purpose

Binary vector for the expression of peroxisome transit peptide fused mCerulean in plants (for Agrobacterium-mediated genetic transformation and visualization of peroxisome.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

Insert

Peroxisome transit peptide fused mCerulean

Expression

Bacterial and Plant

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMS161

Plasmid

#215683

Purpose

Guide only plasmid targeting chrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

U6p::GTCCAGCGGCAGATCGGCGG

Use

CRISPR

Expression

Worm

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMS160

Plasmid

#215682

Purpose

Guide only plasmid targeting chrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

U6p::GTTTGAGTAGAGCACTCAGG

Use

CRISPR

Expression

Worm

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Cox4-V5-APEX2-Ura

Plasmid

#207061

Purpose

Expressing Cox4-V5-APEX2. Marker for mitochondrial inner membrane. Tends to generate facture in thin resin sections.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

V5-APEX2

Expression

Yeast

Promoter

pCOX4

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Cox4-GFP-APEX2-Ura

Plasmid

#207062

Purpose

Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

GFP-APEX2

Expression

Yeast

Promoter

pCOX4

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pERG6-Pex3-4V5-APEX2-URA

Plasmid

#207068

Purpose

Moderate overexpression of Pex3-4V5-APEX2 under ERG6 promoter. Marker for peroxisomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pERG6

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pLSP1-Pex3-4V5-APEX2-URA

Plasmid

#207069

Purpose

Moderate overexpression of Pex3-4V5-APEX2 under LSP1 promoter. Marker for peroxisomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pLSP1

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Tgl3-4V5-APEX2-URA

Plasmid

#207059

Purpose

Expressing Tgl3-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Tgl3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pTGL3

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Pex3-4V5-APEX2-URA

Plasmid

#207057

Purpose

Expressing Pex3-4V5-APEX2. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

PEX3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pPEX3

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHB1

Plasmid

#204668

Purpose

pUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructs

Depositor

Mark Mandel

Article

Burgos et al mSystems. 2020 Dec 15;5(6):e00846-20. do

Insert

FRT-erm-FRT

Use

Synthetic Biology

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pTPI1-Emc1-GFP-APEX2-Ura

Plasmid

#207074

Purpose

Expressing Emc1-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Emc1

Tags

GFP-APEX2

Expression

Yeast

Promoter

pTPI1

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pTPI1-Cox4-GFP-APEX2-Ura

Plasmid

#207075

Purpose

Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

GFP-APEX2

Expression

Yeast

Promoter

pTPI1

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLCS106

Plasmid

#209359

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal moxMaple fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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