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MTS-FUS(IDR)-mCh-CRY2olig

Plasmid

#254612

Purpose

Expresses mt-optoIDR with FUS (1-214 aa) to optogenetically induce biomolecular condensates within mitochondrial matrix in living cells.

Depositor

Marina Feric

Publication

Aththawala Gedara et al Commun Biol. 2026 Apr 23. doi: 10.1038/s

Insert

FUS (FUS Human)

Use

Synthetic Biology

Tags

mCherry-Cry2olig

Expression

Mammalian

Available since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

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MTS-GRSF1(IDR)-mCh-CRY2olig

Plasmid

#254614

Purpose

Expresses mt-optoIDR with GRSF1(1-150 aa) to optogenetically induce biomolecular condensates within mitochondrial matrix in living cells.

Depositor

Marina Feric

Publication

Aththawala Gedara et al Commun Biol. 2026 Apr 23. doi: 10.1038/s

Insert

GRSF1 (GRSF1 Synthetic, Human)

Use

Synthetic Biology

Tags

mCherry-Cry2olig

Expression

Mammalian

Available since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

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pESUMO-RsmB-4AAYBBR

Plasmid

#250650

Purpose

Plasmid for expression of E.coli methyltransferase protein RsmB tagged after 4 amino acids with a YBBR tag

Depositor

Olivier Duss

Publication

Gor et al Sci Adv. 2026 Mar 20;12(12):eaec4037. do

Insert

RsmB (rsmB E. Coli)

Tags

YBBR

Expression

Bacterial

Mutation

YBBR tag inserted after the 4th Amino acid from t…

Promoter

T7

Available since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pHR-HaloTag-P2A-RSV M2-1-C-terminal-1xSunTag

Plasmid

#246867

Purpose

Express HaloTag-P2A-RSV M2-1-C-terminal-1xSunTag (M2-1exo-SunTag) for RSV imaging.

Depositor

Marvin Tanenbaum

Publication

Ratnayake et al Nature. 2026 Jan 28. doi: 10.1038/s41586

Insert

HaloTag-P2A-RSV M2-1-C-terminal-1xSunTag

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Mutation

The RSV M2-1 protein with a C-terminal insertion …

Promoter

SFFV

Available since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1a-EGFP-miR-MCS (JDW 1478)

Plasmid

#242580

Purpose

An EF1a driven expression vector containing an EGFP fused to a microRNA multiple cloning site.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP intron and miR-MCS (empty)

Expression

Mammalian

Promoter

human EF1a

Available since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(E49TAG,G42H,K46H)-mCherry

Plasmid

#241292

Purpose

Mammalian expression of syntaxin-1(E49TAG,G42H,K46H) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Publication

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E49TAG,G42H,K46H

Promoter

CMV

Available since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(E53TAG,G42H,K46H)-mCherry

Plasmid

#241294

Purpose

Mammalian expression of syntaxin-1(E53TAG,G42H,K46H) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Publication

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E53TAG,G42H,K46H

Promoter

CMV

Available since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(I233A,R210H,K84TAG)-mCherry

Plasmid

#241300

Purpose

Mammalian expression of syntaxin-1(I233A,K84TAG,R210H) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Publication

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

I233A,K84TAG,R210H

Promoter

CMV

Available since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Syntaxin1A(K264A,K265A,K84TAG)-mCherry

Plasmid

#241301

Purpose

Mammalian expression of syntaxin-1(K264A,K265A,K84TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Publication

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

K264A,K265A,K84TAG

Promoter

CMV

Available since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pB-TetOn-DEST-EFS-mODC-rtTA-IRES-NEO (JDW 931)

Plasmid

#242578

Purpose

PiggyBac transposon flanked, Tet-on, gateway destination vector.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attR1-CmR-ccdB-attR2

Expression

Mammalian

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pME-B-catenin-S33Y-FLAG (JDW 419)

Plasmid

#242565

Purpose

Gateway middle entry clone containing dominant active B-catenin (S33Y beta catenin)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

S33Y B-catenin (CTNNB1 Human)

Use

Gateway Cloning

Available since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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p-HITI-F9-Ckm

Plasmid

#226120

Purpose

HITI insert construct with Ckm gRNA and hF9 transgene

Depositor

Christopher Nelson

Publication

Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

Inserts

F9 (F9 Human)
Ckm gRNA (Ckm Mouse)

Use

CRISPR

Expression

Mammalian

Available since

Aug. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pUBC(k)-AT5G02100-mKOk

Plasmid

#224848

Purpose

Plasmid for expression of AT5G02100.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Publication

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT5G02100.1 (UNE18 Mustard Weed)

Expression

Plant

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-LexA-oMDC-pA-LexOp-nlsGFP-pA (JDW 1381)

Plasmid

#229838

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexAOp

Use

Tol2 based expression vector

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-LAP

Plasmid

#222322

Purpose

Synchronize the trafficking of LAP from the ER.

Depositor

Franck Perez

Publication

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAP fused to SBP-EGFP (ACP2 Human)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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3xMS-TR-100-PURO HRD

Plasmid

#207582

Purpose

homologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locus

Depositor

Jens Schmidt

Publication

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences

Tags

3xMS2

Expression

Mammalian

Promoter

SV40

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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R152C USP7 TRAF

Plasmid

#227229

Purpose

Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Publication

Korchak et al bioRxiv [Preprint]. 2026 Apr 28:2026.04.

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

R152C; aa 62-205

Promoter

T7-promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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H111Q USP7 TRAF

Plasmid

#227227

Purpose

Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Publication

Korchak et al bioRxiv [Preprint]. 2026 Apr 28:2026.04.

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

H111Q; aa 62-205

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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F118L USP7 TRAF

Plasmid

#227228

Purpose

Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Publication

Korchak et al bioRxiv [Preprint]. 2026 Apr 28:2026.04.

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

F118L; aa 62-205

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSG068

Plasmid

#214259

Purpose

pJN105 with PaftsH2SD and PaFtsH1 ORF cloned between NheI/XbaI

Depositor

Ute Romling

Publication

Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

Insert

Core genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M

Expression

Bacterial

Mutation

18 nucleotide 5' of the start codon (Shine-D…

Promoter

araBAD promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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