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pDONR223_ACD_WT_V5

Plasmid

#83001

Purpose

Gateway Donor vector containing ACD, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ACD (ACD Human)

Use

Gateway entry vector

Mutation

V518A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2IP_p.E463K

Plasmid

#81587

Purpose

Gateway Donor vector containing ERBB2IP , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2IP (ERBIN Human)

Use

Gateway entry vector

Mutation

T271P; E463K; D547G; E1205G; Q1226P; K1287R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.G469S

Plasmid

#82838

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

G469S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_EDNRB_p.F292L

Plasmid

#81402

Purpose

Gateway Donor vector containing EDNRB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EDNRB (EDNRB Human)

Use

Gateway entry vector

Mutation

F292L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DOK1_WT_V5

Plasmid

#82974

Purpose

Gateway Donor vector containing DOK1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

DOK1 (DOK1 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.P367R

Plasmid

#82851

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

P367R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.W450L

Plasmid

#82830

Purpose

Gateway Donor vector containing BRAF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

W450L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_hRNMT_RAM

Plasmid

#213528

Purpose

Expresses the human RNMT-RAM complex

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Inserts

human RNMT (RNMT Human)
RAM

Tags

His6-sumo

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14

Plasmid

#213518

Purpose

Expresses NSP14 of SARS-CoV-2

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

N-terminal His6-sumo

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-NRBP1-Cterm-N-FLAG

Plasmid

#222678

Purpose

overexpressing C-terminus truncation of NRBP1 with N-terminal FLAG

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

NRBP1 (NRBP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

NRBP1 C-terminus truncation

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-TSC22D2-Cterm-N-FLAG

Plasmid

#222684

Purpose

overexpressing C-terminus truncation of TSC22D2 with N-terminal FLAG

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D2 (TSC22D2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

TSC22D2 C-terminus truncation

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.G466A

Plasmid

#81707

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

G466A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.G466E

Plasmid

#81715

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

G466E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CBR3_WT_V5

Plasmid

#83015

Purpose

Gateway Donor vector containing CBR3, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

CBR3 (CBR3 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP2K1_p.K57T

Plasmid

#81695

Purpose

Gateway Donor vector containing MAP2K1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K1 (MAP2K1 Human)

Use

Gateway entry vector

Mutation

K57T; I310N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NRAS_p.Y64D

Plasmid

#81666

Purpose

Gateway Donor vector containing NRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NRAS (NRAS Human)

Use

Gateway entry vector

Mutation

Y64D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FAM173B_WT_V5

Plasmid

#82986

Purpose

Gateway Donor vector containing FAM173B, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

FAM173B (FAM173B Human)

Use

Gateway entry vector

Mutation

T75M

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMAD2_p.D304G

Plasmid

#81615

Purpose

Gateway Donor vector containing SMAD2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMAD2 (SMAD2 Human)

Use

Gateway entry vector

Mutation

D304G

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.R222L

Plasmid

#82922

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

R222L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RB1_p.R451H

Plasmid

#81595

Purpose

Gateway Donor vector containing RB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RB1 (RB1 Human)

Use

Gateway entry vector

Mutation

R451H

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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