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pET-20b-T7-Ntx-K49

Plasmid

#58715

Purpose

Expresses in Escherichia coli T7-Notexin with the amino acid substitution D49K

Depositor

Fiorella Tonello

Publication

Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

Insert

Notexin

Tags

T7 peptide tag

Expression

Bacterial

Mutation

Changed Aspartic Acid 49 to Lysine

Promoter

T7

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pC-0

Plasmid

#124421

Purpose

Full MCS (step 2)

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pISH-Trpm5

Plasmid

#105993

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Trpm5 (Trpm5 Mouse)

Use

To generate riboprobe

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNMHCII-C1-GFP

Plasmid

#46041

Depositor

Robert Adelstein

Publication

Jana et al J Biol Chem. 2006 Aug 25;281(34):24662-7

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

nonmuscle myosin heavy chain II-C, isoform C1 (Myh14 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV immediate early

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only
pYPQ-dpcoCas9-Act3.0

Plasmid

#158408

Purpose

CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

Depositor

Yiping Qi

Publication

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMOS009: pLX304-QuasAr2

Plasmid

#62985

Purpose

Lentivirus production of QuasAr2 fluorescent reporter

Depositor

Adam Cohen

Publication

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr2-mOrange

Use

Gateway Cloning and Lentiviral

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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HCT1_rAAV-eHGT_1137m-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#223900

Purpose

Direct-expressing SYFP2 AAV virus

Depositor

Tanya Daigle, Allen Institute for Brain Science

Publication

Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

Insert

SYFP2

Use

AAV

Mutation

N/A

Promoter

minBglobin

Available since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE33

Plasmid

#90375

Purpose

AP1 - c-Fos, c-Jun, ATF, and JDP gene family gene reporter

Depositor

Ramnik Xavier

Publication

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

AP1

Available since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD1

Plasmid

#195839

Purpose

Expresses human FZD1 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Publication

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FZD1 (FZD1 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMRE-Tn7-165

Plasmid

#118577

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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His-SUMO-Rad23A_pET11a

Plasmid

#169124

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Publication

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hRad23A (RAD23A Human)

Expression

Bacterial

Mutation

wt

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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His-SUMO-hDDI2_pET11a

Plasmid

#169120

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Publication

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Expression

Bacterial

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-150

Plasmid

#118532

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-153

Plasmid

#118535

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE137

Plasmid

#118491

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Expression

Bacterial

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-157

Plasmid

#118571

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE141

Plasmid

#118493

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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PEP108E

Plasmid

#97394

Purpose

binary construct of plasma membrane-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Publication

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Plasma membrane target

Expression

Plant

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYPQ134-tRNA2.0

Plasmid

#158396

Purpose

Golden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Publication

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSF-CjPglB-LpxE

Plasmid

#128389

Purpose

Expression of C. jejuni PglB and F. tularensis LpxE in E. coli

Depositor

Michael Jewett

Publication

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Inserts

PglB oligosaccharylktransferase
LpxE phosphatase

Tags

Flag tag

Expression

Bacterial

Promoter

Ptac and pBAD

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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