We narrowed to 18,716 results for: VARS

1. sgRNA BRCA1 S1363L

   Plasmid

   #139329

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA1 S1363L using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA1 S1363L using base editing
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgRNA BRCA1 E1754K

   Plasmid

   #139330

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA1 E1754K using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA1 E1754K using base editing
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgRNA FANCD2 Q223Stop

   Plasmid

   #139332

   Purpose

   Plasmid expressing a sgRNA to introduce FANCD2 Q223Stop using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert FANCD2 Q223Stop using base editing
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. B-HypaSpCas9

   Plasmid

   #126763

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-HypaSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N692A; M694A; Q695A; H698A; amino acids 1005-1013…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-FLAG-B-eSpCas9

   Plasmid

   #126772

   Purpose

   Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A; K1003A; R1060A; amino acids 1005-1013 repl…

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-Flag-SpCas9-HF1 (without sgRNA; with silent mutations)

   Plasmid

   #126755

   Purpose

   Expression plasmid for human codon-optimized increased fidelity SpCas9-HF1 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. hKCNB1 R312H

   Plasmid

   #124488

   Purpose

   missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

   Depositor

   Federico Sesti

   Article

   Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

   Insert

   human KCNB1 (KCNB1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed arginine 312 to histidine

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIE-N-hα2M-H6

   Plasmid

   #128493

   Purpose

   Expresses human N-α2M (from S24 to E908) in insect cells. With C-t H6x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   A2M (S24-E908) (A2M Human)
   

   Tags

   His tag (6x)

   Expression

   Insect

   Promoter

   IE1

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pS6-N-hα2M-H6

   Plasmid

   #128496

   Purpose

   Expresses human N-α2M (from G27 to A788) in mammalian cells. With C-t H6x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   A2M (G27-A788) (A2M Human)
   

   Tags

   His tag (6x)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. hKCNB1 G379R

    Plasmid

    #124489

    Purpose

    missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

    Depositor

    Federico Sesti

    Article

    Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

    Insert

    human KCNB1 (KCNB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed glycine at 379 to arginine

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-mRIN2 dRA/PINCO

    Plasmid

    #108938

    Purpose

    Retroviral expression of mRIN2 dRA

    Depositor

    Guido Serini

    Article

    Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

    Insert

    Ras and Rab interactor 2 (Rin2 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletion of RA domain (aa 751-836)

    Promoter

    LTR

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-mRIN2 dRHVPS9/PINCO

    Plasmid

    #108939

    Purpose

    Retroviral expression of mRIN2 dRHVPS9

    Depositor

    Guido Serini

    Article

    Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

    Insert

    Ras and Rab interactor 2 (Rin2 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletion of RHVPS9 domain (aa 455-738)

    Promoter

    LTR

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19-Q6KIQ7

    Plasmid

    #103128

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    Q6KIQ7(Cas9 coding gene from Mycoplasma mobile (strain ATCC 43663 / 163K / NCTC 11711))
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-Q8DTE3

    Plasmid

    #103129

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    Q8DTE3(Cas9 coding gene from Treponema denticola (strain ATCC 35405 / CIP 103919 / DSM 14222))
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-D3UFL8

    Plasmid

    #103095

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    D3UFL8(Cas9 coding gene from Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198))
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHH0103_FRK-1/1

    Plasmid

    #91549

    Purpose

    Protein expression and purification of human SH3 domain construct FRK-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    FRK-1/1 (FRK Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHH0103_ITSN1-1/5-IS3/3

    Plasmid

    #91560

    Purpose

    Protein expression and purification of human SH3 domain construct ITSN1-1/5-IS3/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ITSN1-1/5-IS3/3 (ITSN1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHH0103_CACNB1-1/1

    Plasmid

    #91563

    Purpose

    Protein expression and purification of human SH3 domain construct CACNB1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CACNB1-1/1 (CACNB1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103_DLG3-1/1

    Plasmid

    #91525

    Purpose

    Protein expression and purification of human SH3 domain construct DLG3-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    DLG3-1/1 (DLG3 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_FGR-1/1

    Plasmid

    #91543

    Purpose

    Protein expression and purification of human SH3 domain construct FGR-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    FGR-1/1 (FGR Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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