We narrowed to 25,057 results for: Nes

1. pMOD_A0103

   Plasmid

   #91000

   Purpose

   Module A, Promoter: CmYLCV, Gene: AtCas9, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CmYLCV

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Mammalian_SpCas9_Target-AID2S

   Plasmid

   #188648

   Purpose

   Expresses SpCas9_Target-AID2S in mammalian cells

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Insert

   nCas9-AID2S-UGI
   

   Use

   Tags

   UGI

   Expression

   Mammalian

   Mutation

   D10A for SpCas9

   Promoter

   pCMV

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-myc-Ataxin3Q84

   Plasmid

   #22124

   Depositor

   Henry Paulson

   Article

   Chai et al J Neurosci. 1999 Dec 1. 19(23):10338-47.

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pPBbsr2-Lifeact-miRFP703

   Plasmid

   #178530

   Purpose

   Expresses Lifeact-miRFP703 in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   Lifeact-miRFP703
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-scFvGCN4sfGFP-VP64-GB1

   Plasmid

   #141417

   Purpose

   Express scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activation

   Depositor

   Izuho Hatada

   Article

   Morita et al Int J Mol Sci. 2020 Feb 25;21(5). pii: i

   Insert

   scFvGCN4sfGFP-VP64-GB1
   

   Use

   Tags

   HA and sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon

   Plasmid

   #69906

   Purpose

   Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ciRS7 (CDR1-AS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMA-SpCas9-g6

   Plasmid

   #80789

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pH6HTC_HSPA5-Halo

   Plasmid

   #175326

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   HSPA5 (HSPA5 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pluc2-iRFP720

   Plasmid

   #98887

   Purpose

   Mammalian expression

   Depositor

   Laura Mezzanotte

   Article

   Mezzanotte et al Cell Transplant. 2017 Apr 26. doi: 10.37

   Insert

   Firefly Luciferase
   

   Use

   Tags

   iRFP720

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA

    Plasmid

    #176630

    Purpose

    Integrated in CHO-K1 to make parental polyclonal lines for all other MultiFate cell lines

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    EF1a-TET3G-P2A-ERT2-Gal4-BGHpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pQTEV-FLJ30046

    Plasmid

    #34828

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    FLJ30046 (SLAIN1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-DUET-1-mRhubarb713-HO

    Plasmid

    #141199

    Purpose

    Expresses mRhubarb713 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb713
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, W309R, Q310A compared to iRFP

    Promoter

    T7

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. E12-pCLBabe

    Plasmid

    #20918

    Depositor

    Stephen Tapscott

    Article

    Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

    Insert

    E12 (TCF3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G155D

    Promoter

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. E47 2/5-pCS2

    Plasmid

    #20914

    Depositor

    Stephen Tapscott

    Article

    Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

    Insert

    E47 2/5 (TCF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

15. Mammalian_SpCas9_Target-AID3S

    Plasmid

    #188649

    Purpose

    Expresses SpCas9_Target-AID3S in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Insert

    nCas9-AID3S-UGI
    

    Use

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SpCas9

    Promoter

    pCMV

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-Lifeact_Halo_T2A_EGFP

    Plasmid

    #135445

    Purpose

    Mamalian cell expression of HaloTag7 localized at F-actin and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

    Insert

    Lifact-HaloTag7 and EGFP
    

    Use

    Tags

    Lifact

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-C1 beta-catenin Y654F

    Plasmid

    #16072

    Depositor

    Erin Schuman

    Article

    Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

    Insert

    beta-catenin (CTNNB1 Chicken)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Y654F, *(see below)

    Promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSV Sport SREBP-1c dom neg

    Plasmid

    #8885

    Depositor

    Bruce Spiegelman

    Article

    Kim et al Genes Dev. 1996 May 1. 10(9):1096-107.

    Insert

    SREBP-1c (Srebf1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y305A amino acid substitution in the active versi…

    Promoter

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF6 VPS52-V5-6His

    Plasmid

    #175096

    Purpose

    V5 tagged VPS52 is expressed in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

    Insert

    VPS52 (VPS52 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    N666D- please see depositor comment

    Promoter

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRIPZ-NLS-PTEN

    Plasmid

    #206984

    Purpose

    Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with NLS

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on promoter

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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