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pRSET YBX-1

Plasmid

#13035

Depositor

Peter Newburger

Article

Shen et al J Biol Chem. 1998 Mar 6. 273(10):5443-6.

Insert

YBX1 (YBX1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only
pCk3

Plasmid

#136697

Purpose

Acceptor plasmid for odd level position 3, lacZ blue-white screening. Part of the OpenPlant toolkit.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBWB419

Plasmid

#140637

Purpose

RSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

Cas9

Expression

Bacterial

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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pTC217

Plasmid

#70018

Purpose

Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (Cas9/sgRNA) + Donor + GVR

Use

CRISPR

Expression

Plant

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRRL_U2AF1_S34F_mCherry

Plasmid

#84019

Purpose

expression of mutant U2AF1 in mammalian cells

Depositor

Robert Bradley

Article

Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

Insert

U2AF1 (U2AF1 Human)

Use

Lentiviral

Tags

FLAG and T2A-mCherry

Expression

Mammalian

Mutation

S34F

Promoter

PGK

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pFW1000

Plasmid

#224210

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCk2

Plasmid

#136696

Purpose

Acceptor plasmid for odd level position 2, lacZ blue-white screening. Part of the OpenPlant toolkit.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pTalpha1-iCre

Plasmid

#133924

Purpose

Neuron-specific expression of iCre

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

iCre

Expression

Mammalian

Mutation

Codon optimized

Promoter

neuron-specific tubulin-alpha-1 promoter

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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p6641 MSCV-P C-FlagHA 18E7-Kozak

Plasmid

#35019

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV18 E7 (E7 HPV 18)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

March 14, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA4-Beclin1-HA

Plasmid

#24399

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

HA

Expression

Mammalian

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Cdc45

Plasmid

#211426

Purpose

mammalian expression of human Cdc45 C-terminally fused to EGFP

Depositor

Helmut Pospiech

Article

Kohler et al Cell Cycle. 2016;15(7):974-85. doi: 10.1

Insert

Cdc45 (CDC45 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-CamKII-PSD95.FingR-eGFP-CCR5TC

Plasmid

#126218

Purpose

Global labeling of excitatory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

Lentiviral

Expression

Mammalian

Promoter

CamKIIa

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBXNH3CA_MBP

Plasmid

#132700

Purpose

expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Maltose binding Protein

Tags

Avi and His

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET-6His-MBP-MpAgo

Plasmid

#79249

Purpose

This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression

Depositor

Jennifer Doudna

Article

Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p

Insert

Marinitoga piezophila Argonaute

Use

CRISPR

Tags

6XHis-tag + MBP + PreScission Cleavage Site

Expression

Bacterial

Promoter

T7

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMOS023E: Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

Plasmid

#163077

Purpose

Entry vector for: Peredox NADH/NAD+ sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Peredox

Use

Gateway entry vector

Tags

mCherry

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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MAC_C_INSR

Plasmid

#187772

Purpose

MAC-tagged gene expression of human INSR

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

INSR (INSR Human)

Expression

Mammalian

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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Calreticulin-SuperNova-KDEL

Plasmid

#131857

Purpose

Expresses SuperNova fused to ER protein calreticulin and KDEL ER-retention signal

Depositor

Geert van den Bogaart

Article

Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

Insert

SuperNova

Tags

KDEL ER-retention signal and Signal sequence of h…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ING2

Plasmid

#13294

Depositor

Curtis Harris

Article

Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

Insert

ING2 (INGX Human)

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Orai1

Plasmid

#21638

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai1 (ORAI1 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pMAH-POLY

Plasmid

#64915

Purpose

Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

Depositor

Huiwang Ai

Article

Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

Inserts

Polyspecific aminoacyl-tRNA Synthetase
Amber Suppression tRNA

Tags

None

Expression

Mammalian

Promoter

CMV and U6/H1

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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