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psiCHECK-2-Ywhag-3'UTR

Plasmid

#61778

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhag

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhag 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pENTR221-WNT3A

Plasmid

#79501

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3A CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WNT3A (WNT3A Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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HL 2818 (=HL 865 + pHL 489)

Plasmid

#30032

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:DsrA, pLlacO-1:hfq, pLtetO-1:rpoS::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only
pAct:MCP-VP64

Plasmid

#78904

Purpose

Expresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2:VP64

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

March 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-CaMKKalpha RP deletion

Plasmid

#33328

Depositor

Anthony Means

Article

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Deleted RP domain (amino acids 167-189 missing)

Promoter

SV40

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only
Dom34(1-138)

Plasmid

#19101

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Tags

C tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pSR21

Plasmid

#69156

Purpose

read-outlox2272 mCherry to tagBFP switch for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
TagBFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

rps-27

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pEBG-TFII-deltaNLS1

Plasmid

#22153

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Mutation

deletion of amino acids 297-304

Available Since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pHL 1011

Plasmid

#52993

Purpose

pLlac0-1:RhyB-mCherry, pLtetO-1:RBS (st7) gfp

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

RhyB sRNA
RBS (st7)

Use

Synthetic Biology

Tags

GFP and RBS-mCherry

Expression

Bacterial

Promoter

pLlac0-1 and pLtetO-1

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Xbp1-3'UTR

Plasmid

#61779

Purpose

Luciferase reporter containing the 3'UTR of mouse Xbp1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Xbp1 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pP2A11 (GB0082)

Plasmid

#68168

Purpose

Provides the S. Lycopersicum fruit promoter 2A11 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Sl2A11 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA3 WT

Plasmid

#66179

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 WT

Plasmid

#66175

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-CaMKKbeta rat 1-460 K193A

Plasmid

#33326

Depositor

Anthony Means

Article

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

truncated at 460, K193A (S3V, G96R, and P108L als…

Promoter

SV40

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat 1-460 D311A

Plasmid

#33325

Depositor

Anthony Means

Article

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

truncated at 460, D311A (S3V, G96R, and P108L als…

Promoter

SV40

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
p7012 MSCV-IP N-HAonly 18E6nonsplice

Plasmid

#44148

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV18E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Internal splice site mutated

Promoter

MSCV LTR

Available Since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
HL 4218 (=HL 716 + pHL 1411)

Plasmid

#30105

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:DsrA, pCon:fhlA:T7noRBS(900bps), pLlacO-1:rpoS::gfp

Use

Synthetic Biology

Tags

T7noRBS, GFP

Expression

Bacterial

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
HL 4212 (=HL 716 + pHL 1414)

Plasmid

#30104

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pCon:fhlA::T7noRBS(900bps), pLlacO-1:rpoS::gfp

Use

Synthetic Biology

Tags

T7noRBS, GFP

Expression

Bacterial

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only
GUCY1B3

Plasmid

#39121

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

GUCY1B3 (GUCY1B1 Human)

Tags

TEV-His6-Flag

Expression

Bacterial

Mutation

This is a complex of two proteins; GUCY1A3 (aa 46…

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pGS1044

Plasmid

#63797

Purpose

Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal 3xFLAG tag and a LEU2 selection marker

Depositor

Monika Golas, Bjoern Sander

Article

Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1

Insert

3xFLAG followed by KlLEU2

Use

E. coli cloning vector

Tags

3xFLAG

Promoter

none

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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