We narrowed to 15,981 results for: LEA

1. IPCEF1 (PIP3EA-c002)

   Plasmid

   #98240

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PIP3EA (IPCEF1 Human)
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJExpress414_GmPOPB-W695del

   Plasmid

   #92241

   Purpose

   Express W695-deletion mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-W695deletion mutant
   

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Tryptophan 695 deleted

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJExpress414_GmPOPB-R663Q

   Plasmid

   #92240

   Purpose

   Express R663Q mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-R663Q mutant
   

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Arginine 663 to Glutamine

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJExpress414_GmPOPB-H698A

   Plasmid

   #92237

   Purpose

   Express H698A mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-H698A mutant
   

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Histidine 698 to Alanine

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJExpress414_GmPOPB-R663A

   Plasmid

   #92238

   Purpose

   Express R663A mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-R663A mutant
   

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Arginine 663 to Alanine

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEHIShqprtK139A

   Plasmid

   #92403

   Purpose

   hQPRTase harbouring K139A mutation

   Depositor

   Huanting Liu, James Naismith

   Article

   Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

   Insert

   hQPRTase (QPRT Human)
   

   Tags

   TEV cleacable 6xHis

   Expression

   Bacterial

   Mutation

   K139A

   Promoter

   T7

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a - TryX

   Plasmid

   #89623

   Purpose

   [Edit] Expresses recombinant 6HIS tagged T. cruzi tryparedoxin protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Tryparedoxin
   

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR-IAA8

   Plasmid

   #83873

   Purpose

   Gateway donor vector for IAA8

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   IAA8 (IAA8 Mustard Weed)
   

   Use

   Gateway donor vector

   Available Since

   Oct. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR-IAA16

   Plasmid

   #83854

   Purpose

   Gateway donor vector for IAA16

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   IAA16 (IAA16 Mustard Weed)
   

   Use

   Gateway donor vector

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDGE63

    Plasmid

    #79445

    Purpose

    recipient plasmid [single nuclease]; p35S:Cas9, BASTA

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    ccdB (ccdB(letD) )
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    BsaI site eliminated

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. 4QL1

    Plasmid

    #61942

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WDR5:APC041-G05:C36415 (WDR5 Human)
    

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pAGM1467

    Plasmid

    #50298

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    5'UTR, omega (Tobacco Mosaic Virus) + HIS tag + enterokinase cleavage site
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. GMIP

    Plasmid

    #32046

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    GMIP (GMIP Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 80-357 only

    Available Since

    Jan. 13, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. Sgf73(1-106)/PET32a

    Plasmid

    #26962

    Depositor

    Cynthia Wolberger

    Article

    Samara et al Science. 2010 May 21. 328(5981):1025-9.

    Insert

    Sgf73(1-106) (SGF73 Budding Yeast)
    

    Tags

    His6 and Trx

    Expression

    Bacterial

    Mutation

    with TEV site to cleave the N-terminal his+Trx tag

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pGEM-7Zf-wild

    Plasmid

    #23160

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Ribonuclease assay sub1
    

    Use

    In vitro transcription

    Available Since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pGEM-7Zf-deleted

    Plasmid

    #23161

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Ribonuclease assay sub2
    

    Use

    In vitro transcription

    Available Since

    June 4, 2010

    Availability

    Academic Institutions and Nonprofits only

17. CJ47 (U1 snRNA (U1;21d) 3G mutant)

    Plasmid

    #17776

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    U1 snRNA (U1;21d) (snRNA:U1:21D Fly)
    

    Mutation

    3G mutant

    Available Since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

18. M15 pCS-twhh-nBeta gal

    Plasmid

    #17152

    Depositor

    Randall Moon

    Article

    Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

    Insert

    twhh promoter (shhb Zebrafish)
    

    Tags

    nuclear beta-gal

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pCpSRb-4

    Plasmid

    #13726

    Depositor

    Thomas Schwartz

    Article

    Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

    Insert

    Signal recognition particle receptor β subunit (SRP102 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Circularly permuted SRβD210K183 with Precission p…

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLIC.B3.MJ0130m

    Plasmid

    #11528

    Depositor

    Sung-Hou Kim

    Article

    Kim et al Proc Natl Acad Sci U S A. 2005 Mar 1. 10

    Insert

    Type I restriction-modification enzyme (MJ_0130m M. jannaschii)
    

    Tags

    HisTag, TEV cleavable

    Expression

    Bacterial

    Available Since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only