We narrowed to 19,373 results for: LEA

1. Sema3a(S)-Fc-His

   Plasmid

   #72138

   Purpose

   Expresses the Sema3A protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3a (Sema3a Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDNA_B2AR-no-pep

   Plasmid

   #47439

   Purpose

   control FRET sensor for beta2-adrenergic receptor

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

   Insert

   beta2-adrenergic receptor (ADRB2 Human)
   

   Use

   Tags

   10 nm ER/K helix, HA, mCerulean, mCitrine, and no…

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Sept. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

3. LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

   Plasmid

   #47455

   Purpose

   LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   NLS(alpha-imp)-CRY2PHR-NLS-VP64
   

   Use

   AAV

   Tags

   2A_GFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. D28 Cortactin GFP

   Plasmid

   #26723

   Depositor

   Anna Huttenlocher

   Article

   Perrin et al Mol Biol Cell. 2006 Jan . 17(1):239-50.

   Insert

   Cortactin (Cttn Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion removed 28 amino acids (aa# 334-361) enc…

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pCDNA_B2AR-q-pep

   Plasmid

   #47437

   Purpose

   FRET sensor for beta2-adrenergic receptor

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

   Insert

   beta2-adrenergic receptor (ADRB2 Human)
   

   Use

   Tags

   10 nm ER/K helix, HA, mCerulean, mCitrine, and q-…

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Sept. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pGEX-6p-hDcp2 E147/8Q

   Plasmid

   #72217

   Purpose

   bacterial expression of GST-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

   Depositor

   Megerditch Kiledjian

   Article

   Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

   Insert

   Dcp2 (DCP2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   E147Q, E148Q and F404L (please see depositor'…

   Promoter

   tac

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

7. E12-cTAP

   Plasmid

   #20916

   Depositor

   Stephen Tapscott

   Article

   Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

   Insert

   E12 cTAP (Tcf3 Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   TAP tag is fused to the 3' end of the E12 ge…

   Promoter

   Available since

   May 15, 2009

   Availability

   Academic Institutions and Nonprofits only

8. PA-mCherry1-LaminB1-10

   Plasmid

   #57186

   Purpose

   Localization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Article

   York et al Nat Methods. 2011 Apr;8(4):327-33. doi:

   Insert

   LaminB1 (LMNB1 Human)
   

   Use

   Tags

   PA-mCherry1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

9. Sema3a(L, del13)-AP-His

   Plasmid

   #72011

   Purpose

   Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 13), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3a (Sema3a Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-FLEX-tmeGFP-IRES-nls-mRuby2

    Plasmid

    #63060

    Purpose

    Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    nls-mRuby2
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mTFP1-LaminB1-7

    Plasmid

    #55492

    Purpose

    Localization: Nuclear Envelope, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

12. PRK5-FLAG-USP13/5-6

    Plasmid

    #61748

    Purpose

    express N-terminally FLAG tagged human chimera USP13/5-6

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    USP13/5-6 chimera (USP5, USP13 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    USP13 (1-721aa) fused to USP5 (717-859aa); D23Y i…

    Promoter

    CMV

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mTFP1-LaminB1-10

    Plasmid

    #55493

    Purpose

    Localization: Nuclear Envelope, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

14. Sema3f(L,+)-Fc-His

    Plasmid

    #72147

    Purpose

    Expresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains no deletion in exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3f (Sema3f Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Sema3a(L, del3)-AP-His

    Plasmid

    #72010

    Purpose

    Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3a (Sema3a Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCS2 Notch1 LNG-6MT

    Plasmid

    #41738

    Depositor

    Jeffrey Nye, Raphael Kopan

    Article

    Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

    Insert

    Notch-1 Lin-Notch-glp (LNG) repeat-containing construct (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Contains murine Notch-1 leader peptide (aa 1-23) …

    Promoter

    Available since

    Jan. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

17. Sema3b(L)-AP-His

    Plasmid

    #72013

    Purpose

    Expresses the Sema3B protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3b (Sema3b Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Sema3a(S)-AP-His

    Plasmid

    #72012

    Purpose

    Expresses the Sema3A protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3a (Sema3a Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 3xFN-Tel-TAL/pMXs-puro

    Plasmid

    #63590

    Purpose

    Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector with the puromycin resistance gene.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038

    Insert

    3xFVN-Tel-TAL
    

    Use

    Retroviral and TALEN

    Tags

    3xFLAG-tag, V5-tag, and nuclear localization sign…

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. D998-EGFR-ET1

    Plasmid

    #44186

    Depositor

    Timothy Springer

    Article

    Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

    Insert

    D998-EGFR-ET1 (EGFR Human)
    

    Use

    Tags

    3C cleavge site, protein C peptide tag, his6, SBP…

    Expression

    Mammalian

    Mutation

    EGFR amino acids 1−998

    Promoter

    CVM

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only