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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,036 results for: his

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Showing: 11361 - 11380 of 23036 results

  3. pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

    Plasmid
    #101214
    Purpose
    Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/K855A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C and K855A
    Promoter
    T7
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    101214-plasmid-map-sequence-id-197223

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  15. pNC2

    Plasmid
    #72438
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)
    Depositor
    T. J. Kappock, JoAnne Stubbe
    Article
    Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Mutation
    Pro64 to Arg; Asp65 to His; Gln206 to Arg
    Promoter
    T7 and n/a
    Available Since
    May 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72438-plasmid-map-sequence-id-140433

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Showing: 11361 - 11380 of 23036 results