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pAG227 CORO1C 3'UTR mut

Plasmid

#12030

Depositor

David Bartel

Publication

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

CORO1C 3'UTR mut (CORO1C Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pAG81 ZAP3 3'UTR mut

Plasmid

#12057

Depositor

David Bartel

Publication

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N3 3'UTR mut (YLPM1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pMVP-blast-mCherry-LGALS8

Plasmid

#251003

Purpose

Lentiviral vector with constitutive expression of mCherry tagged human galectin 8

Depositor

Jörn Coers

Publication

Dickinson et al Proc Natl Acad Sci U S A. 2026 Sep;123(3

Insert

galectin 8 (LGALS8 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

EF-1a

Available since

Aug. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mEmerald-ER WPRE

Plasmid

#236230

Purpose

AAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulum

Depositor

Jennifer Lippincott-Schwartz

Publication

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mEmerald-PRL signal peptide-KDEL sequence (PRL Bovine)

Use

AAV

Tags

mEmerald

Promoter

human Synapsin 1

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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BIC-Gag-DDX3

Plasmid

#233687

Purpose

Expresses a fusion between the Murine Leukemia Virus Gag polyprotein and the human DDX3 protein to produce virus-like particles loaded with DDX3 and deliver it to target cells.

Depositor

Emiliano Ricci

Publication

Labaronne et al Nat Commun. 2025 Feb 18;16(1):1706. doi:

Insert

DEAD-box helicase 3 (DDX3X Human)

Use

Retroviral

Tags

Gag (Murine Leukemia Virus)

Expression

Mammalian

Promoter

hCMV

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgIl1r1_#2-hygro

Plasmid

#231990

Purpose

Knockout mouse Il1r1

Depositor

Brian Ruffell

Publication

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance (Il1r1 Mouse)

Use

CRISPR and Lentiviral

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgRipk1_#1-puro

Plasmid

#231984

Purpose

Knockout mouse Ripk1

Depositor

Brian Ruffell

Publication

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)

Use

CRISPR and Lentiviral

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgRipk1_#2-puro

Plasmid

#231983

Purpose

Knockout mouse Ripk1

Depositor

Brian Ruffell

Publication

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)

Use

CRISPR and Lentiviral

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgCasp8_#2-puro

Plasmid

#231978

Purpose

Knockout mouse Casp8

Depositor

Brian Ruffell

Publication

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)

Use

CRISPR and Lentiviral

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgCasp8_#1-puro

Plasmid

#231977

Purpose

Knockout mouse Casp8

Depositor

Brian Ruffell

Publication

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)

Use

CRISPR and Lentiviral

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-α-cateninΔβH

Plasmid

#229705

Purpose

Transient expression of GFP-alpha-catenin-delta-beta-H in mammalian cells

Depositor

Alpha Yap

Publication

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20 2xHA GPR137

Plasmid

#226813

Purpose

Tet-inducible lentiviral vector for 2xHA GPR137 expression

Depositor

Ramiro Iglesias-Bartolome

Publication

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR137 (GPR137 Human)

Use

Lentiviral

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_001164351

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.BMI1

Plasmid

#224711

Purpose

Expresses N-terminal hexahistidine-tagged human BMI1 (PCGF4) in insect cells.

Depositor

Chen Davidovich

Publication

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

BMI1 (BMI1 Human)

Tags

6x histidine tag

Expression

Insect

Promoter

Polyhedrin

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3−3xFLAG−AGO2(S387A)

Plasmid

#220462

Purpose

Express S387 phospho-null mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Publication

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Serine 387 to alanine

Promoter

CMV

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3−3xFLAG−AGO2(S387D)

Plasmid

#220463

Purpose

Express S387 phosphomimetic mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Publication

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Serine 387 to aspartate

Promoter

CMV

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-p21-HA(dPCNA)

Plasmid

#215112

Purpose

Expresses p21-HA(dPCNA) in mammalian cells from an inducible lentiviral vector.

Depositor

Michele Pagano

Publication

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

p21(dPCNA) denotes mutations M147A, D149A, F150A.

Promoter

CMV (Tet inducible)

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-ARNTL-shRNA3

Plasmid

#166915

Purpose

Lentivirus for inducible knockdown of human ARNTL(BMAL1)

Depositor

Roland Friedel

Publication

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

ARNTL shRNA (BMAL1 Human)

Use

Lentiviral

Expression

Mammalian

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPBpuro-ErbB1(DEL1011)

Plasmid

#197366

Purpose

Encoding human ErbB1 mutant.

Depositor

Michiyuki Matsuda

Publication

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFR mutant. (EGFR Human)

Expression

Mammalian

Mutation

deleted amino acids 1011-1186

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-PAL2

Plasmid

#196691

Purpose

Rep/Cap plasmid for the production of PAL2, an AAV capsid with CNS tropism in macaques.

Depositor

Pardis Sabeti

Publication

Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

PTQGTVR insert between amino acids 588 and 589 of…

Promoter

p41

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-mLepr-COMP5AP-AviTag-9xHis

Plasmid

#157628

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Publication

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

mLepr (Lepr Mouse)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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