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AiP1298 - pAAV-AiE0462m-minBG-iCre-HGHpA

Plasmid

#230619

Purpose

AiE0462m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre

Use

AAV

Promoter

minBG

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pBK1297-AAV-EFSNC-dCjCas9-HP1b(79-173)

Plasmid

#223142

Purpose

Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 79-173

Promoter

EF1a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1295-AAV-EFSNC-dCjCas9-HP1a(72-177)

Plasmid

#223140

Purpose

Expression of truncated HP1a with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 72-177

Promoter

EF1a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsUpf1_115-1129-S1107A-siRNAres_S

Plasmid

#147477

Purpose

Mammalian Expression of HsUpf1_115-1129-S1107A-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsUpf1_115-1129-S1107A-siRNAres (UPF1 Human)

Expression

Mammalian

Mutation

4 non silent mutations E167G, K282R, A442G and T1…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GFP-IRES-hSNCA (S129D)

Plasmid

#185719

Purpose

AAV expression of GFP and human α-Synuclein with S129D mutation from hSyn promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

synuclein, alpha (SNCA Human)

Use

AAV

Mutation

Changed Ser 129 to Asp

Promoter

hSyn

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GFP-IRES-hSNCA (S129A)

Plasmid

#185718

Purpose

AAV expression of GFP and human α-Synuclein with S129A mutation from hSyn promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

synuclein, alpha (SNCA Human)

Use

AAV

Mutation

Changed Ser 129 to Ala

Promoter

hSyn

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHBS1292 TDP43 RRM-GFP G368W+W385G

Plasmid

#107838

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G368W and W385G mutations in LLPS reporter

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-J23151-deGFP-T500 (129p)

Plasmid

#70205

Purpose

Promoter panel

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ11296: pHR-HSP-hyperdCas12a-miniVPR-EPS-puro

Plasmid

#210604

Purpose

heat shock promoter - activated hyperdCas12a-based transcriptional activator

Depositor

Stanley Qi

Article

Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

Insert

hyperdCas12a-miniVPR

Use

CRISPR and Lentiviral

Promoter

truncated hsp70

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ11299: pHR-HSP-ABE8e-hyperdCas12a-EPS-puro

Plasmid

#210605

Purpose

heat shock promoter - activated hyperdCas12a-based transcriptional base editor

Depositor

Stanley Qi

Article

Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

Insert

ABE8e-hyperdCas12a

Use

CRISPR and Lentiviral

Promoter

truncated hsp70

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-CaMKKbeta rat S129A, S133A, S137A

Plasmid

#32456

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S129A, S133A, S137A (S3V, G96R, and P108L also pr…

Promoter

SV40

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat S129D, S133D, S137D

Plasmid

#32457

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S129D, S133D, S137D (S3V, G96R, and P108L also pr…

Promoter

SV40

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
AiP2129 - pAAV-AiE2656m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#243074

Purpose

AiE2656m is an enhancer sequence designed to drive AAV-mediated transgene expression in specific population sof brain cells.

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Insert

SYFP2

Use

AAV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15708 - pAAV-AiE1129m_3xC1-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#243317

Purpose

AiE1129m_3xC1 is an enhancer sequence designed to drive AAV-mediated transgene expression in specific populations of brain cells.

Depositor

Allen Institute for Brain Science, Jonathan Ting

Insert

SYFP2

Use

AAV

Available Since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

Plasmid

#229823

Purpose

A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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AiP20129 - pAAV-AiE3288m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230563

Purpose

AiE3288m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1358 - pAAV-AiE2129m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230530

Purpose

AiE2129m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-Mad3 [N129/15R-rat IgG2a] - Chimeric

Plasmid

#231847

Purpose

Mammalian expression plasmid of anti-Mad3 (Human) rat IgG2a R-mab. Derived from hybridoma N129/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Mad3 (Homo sapiens) recombinant mouse monoclonal antibody (MXD3 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pBK1298-AAV-EFSNC-dCjCas9-HP1bNH(111-173)

Plasmid

#223143

Purpose

Expression of truncated HP1b with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1b (CBX1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 111-173

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1299-AAV-EFSNC-dCjCas9-MBD1(529-592)

Plasmid

#223144

Purpose

Expression of truncated MBD1 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MBD1 (MBD1 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 529-592

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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