We narrowed to 2,693 results for: CCS

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pcDNA3.1 PA-mCit

Plasmid

#113443

Purpose

ProteinA-mCitrine control expression vector

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p97-AE-mycStrep

Plasmid

#31841

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

A232E

Promoter

CMV

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
SOD1_pCSdest

Plasmid

#53879

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pSLIK TT 3xFLAG ID2 neo

Plasmid

#98393

Purpose

Lentiviral vector for inducible expression of human ID2 with 3xFLAG N-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

ID2 (ID2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe JunD-Venus puro

Plasmid

#58496

Purpose

mammalian expression of Venus-tagged mouse JunD

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

JunD (Jund Mouse)

Tags

Venus

Expression

Mammalian

Available Since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-p97-RH-mycStrep

Plasmid

#31839

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

R155H

Promoter

CMV

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pFL-SV40-Rac1 5'-UTR

Plasmid

#115354

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of Rac1

Depositor

Ming-Chih Lai

Article

Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

Insert

Rac1 5'-UTR (RAC1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pX330_rActb sgRNA / hSpCas9

Plasmid

#172833

Purpose

Mammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJMP1274

Plasmid

#119266

Purpose

B. subtilis deltaTn7 site and flanking sequence cloned into pACYC

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

B. subtilis deltaTn7

Expression

Bacterial

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1263

Plasmid

#119264

Purpose

B. subtilis attTn7 site and flanking sequence cloned into pACYC

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

B. subtilis attTn7

Expression

Bacterial

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pX330_rSyn1 sgRNA / hSpCas9

Plasmid

#172840

Purpose

Mammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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MAP3K7CL_pCSdest

Plasmid

#53808

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

MAP3K7CL (C21orf7 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
EZH2-TALEN_Right_No_FLAG

Plasmid

#79900

Purpose

Untagged EZH2-TALEN_Right to target the N-terminus of human EZH2

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

EZH2-TALEN_Right_No_FLAG (EZH2 Human)

Use

TALEN

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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YFP(1-158)-STOP

Plasmid

#66107

Purpose

This vector attaches YFP(1-158) to the C-terminus of a protein

Depositor

Catherine Berlot

Article

Hu et al J Biol Chem. 2013 Apr 19;288(16):11416-3

Insert

YFP(1-158)STOP

Expression

Mammalian

Mutation

Gln69 was changed to Met.

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pFL-SV40-GNB2 5'-UTR

Plasmid

#115355

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of GNB2

Depositor

Ming-Chih Lai

Article

Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

Insert

GNB2 5'-UTR (GNB2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pY036_ATP1A1_G5

Plasmid

#86618

Purpose

Expresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1

Depositor

Yannick Doyon

Article

Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

Insert

ATP1A1 G5 crRNA + AsCpf1-3xHA

Use

CRISPR; Co-selection via hdr using ouabain

Tags

3xHA

Expression

Mammalian

Promoter

CBh

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p97-RG-mycStrep

Plasmid

#31840

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

R95G

Promoter

CMV

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-103)

Plasmid

#60357

Purpose

Express the RRM1 of mouse MSI1 in bacterial cells for protein purification

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-103) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

contains amino acids 7-103 of Msi1

Promoter

T7

Available Since

Nov. 24, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)

Plasmid

#60245

Purpose

Express the RBD of mouse MSI1 in bacterial cells for protein purification

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

contains aa7-192 of Msi1

Promoter

T7

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pET-22HT-MSI1(7-192)R61E

Plasmid

#60353

Purpose

R61E mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R61E; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only