We narrowed to 9,426 results for: ef1

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pRS415-TEF1pr-SEC61-FAPoptim

Plasmid

#221115

Purpose

FAP tagged SEC61 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

SEC61-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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YX022 (PB)EF1a-LIDARsensor (hygR)

Plasmid

#233431

Purpose

EF1a-driven LIDAR reporter RNA

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xMS2))cSG-EGFP

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-STE3-GFP

Plasmid

#221113

Purpose

GFP-tagged STE3 (C-terminally tagged, optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

STE3-GFP

Expression

Yeast

Promoter

TEF1

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-ANP1-FAPoptim

Plasmid

#221120

Purpose

FAP-tagged marker for the cis-Golgi (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

ANP1-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-ERG6-FAPoptim

Plasmid

#221121

Purpose

FAP-tagged marker for lipid droplets (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

ERG6-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-PMA1-FAPoptim

Plasmid

#221122

Purpose

FAP-tagged marker for the plasma membrane (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

PMA1-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-RPA34-FAPoptim

Plasmid

#221123

Purpose

FAP-tagged marker for the nucleus (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

RPA34-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-SEC7-FAPoptim

Plasmid

#221124

Purpose

FAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

SEC7-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-VPH1-FAPoptim

Plasmid

#221125

Purpose

FAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

VPH1-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-TBET-T2A-mCHERRY

Plasmid

#224296

Purpose

Expresses mouse TBET and mCherry under EF1a promoter

Depositor

Nikhil Joshi

Article

Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

Insert

TBET T2A mCherry (Tbx21 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2

Plasmid

#229738

Purpose

Expresses Halo tagged human LRRK2 in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Tags

Halo

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-mSTING (1-339)

Plasmid

#229740

Purpose

Expresses mouse STING amino acids 1-339 in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

STING (Sting1 Mouse)

Tags

None

Expression

Mammalian

Mutation

Contains amino acids 1-339

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHIV-EF1a-Il1a-ZsGreen

Plasmid

#231987

Purpose

Express IL-1alpha-ZsGreen fusion protein

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

IL-1alpha-ZsGreen fusion construct

Use

Lentiviral

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro

Plasmid

#195567

Purpose

PiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cells

Depositor

Joanna Wysocka

Article

Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

Insert

TIR1

Expression

Mammalian

Promoter

Ef1-alpha

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-2O-crScaffold_EF1a-BFP

Plasmid

#224862

Purpose

crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from EF1a promoter

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hU6-2xTetO

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-2O-crBFP_EF1a-BFP

Plasmid

#224783

Purpose

BFP-targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and target BFP protein expressed from EF1a promoter

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

crBFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hU6-2xTetO

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-2O-crEGFP_EF1a-BFP

Plasmid

#224784

Purpose

EGFP targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and non-target BFP protein expressed from EF1a promoter

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

crEGFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hU6-2xTetO

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-crBFP_EF1a-BFP

Plasmid

#224785

Purpose

BFP targeting crRNA for RfxCas13d expressed from hU6 promoter and target BFP protein expressed from EF1a promoter

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

crBFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hU6 and hU6-2xTetO

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-CrEGFP_EF1a-BFP

Plasmid

#224786

Purpose

EGFP targeting crRNA for RfxCas13d expressed from hU6 promoter and non-target BFP protein expressed from EF1a promoter

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

crBFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hU6 and hU6-2xTetO

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-LEF1-Brd 196

Plasmid

#219154

Purpose

Transcription factor LEF1 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

LEF1 (LEF1 Human)

Use

Lentiviral

Mutation

Codon optimized

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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