We narrowed to 3,175 results for: tef

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pMKV001

Plasmid

#35107

Depositor

John McCusker

Publication

Vorachek-Warren et al Yeast. 2004 Jan 30;21(2):163-71.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dsdaMX4

Use

Yeast replicating

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pEasyG3_nat

Plasmid

#184918

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

Depositor

Jeferson Gross

Publication

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pV1329

Plasmid

#111438

Purpose

Solo vector pV1326 +sgCgADE2

Depositor

Gerald Fink

Publication

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgCgADE2

Use

CRISPR

Expression

Yeast

Available since

Dec. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB3049(gRNA XII-4)

Plasmid

#73291

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-FKBP-GFP-HIS3MX6

Plasmid

#183023

Purpose

PCR-based tagging of yeast

Depositor

Santiago Di Pietro

Publication

Lamb et al STAR Protoc. 2022 Apr 14;3(2):101323. do

Insert

FKBP

Use

Yeast genomic targeting

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-HALO-Kanamycin

Plasmid

#188933

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Publication

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-Kanamycin

Expression

Yeast

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)

Plasmid

#173456

Purpose

Contains mScarletH-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Publication

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-H-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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YCplac33-NAT_MCART1

Plasmid

#140591

Purpose

Express MCART1 in S. cerevisiae

Depositor

David Sabatini

Publication

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

SLC25A51 with 5' and 3' UTR of scNDT1 (SLC25A51 Human)

Expression

Yeast

Mutation

codon-optimized for expression in S. cerevisiae

Promoter

NDT1 promoter and 3'UTR

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pV1464

Plasmid

#111440

Purpose

N. castellii Solo CRISPR vector, marked with URA3 and NatR

Depositor

Gerald Fink

Publication

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCB32

Plasmid

#111911

Purpose

Expresses gRNA to target Nte1 locus and has HygR marker

Depositor

Yi Tang

Insert

gRNA cassette targeting Nte1 locus

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

SNR52p

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pH3F

Plasmid

#85773

Purpose

N-ICE plasmid pH3F

Depositor

Scott Briggs

Publication

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-3FLAG

Use

Cre/Lox

Expression

Yeast

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pHB4

Plasmid

#53462

Purpose

This plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.

Depositor

Elizabeth Conibear

Publication

Burston et al J Cell Biol. 2009 Jun 15. 185(6):1097-11

Insert

yeGFP-Snc1-Suc2

Expression

Yeast

Promoter

ADH

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFA6a-link-GFPγ-SpHis5

Plasmid

#60781

Purpose

Yeast C-terminal tagging vector, which creates gene fusions with GFPγ using SpHis5 selection

Depositor

Linda Huang

Publication

Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

Insert

GFPγ

Use

Yeast integrating

Mutation

T65C S72A V163A I167T L231H

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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SIV URA PCP-NLS-GFPEnvy

Plasmid

#189941

Purpose

Single-integration vector for inserting PCP-NLS-GFPEnvy at the URA3 locus in Saccharomyces cerevisiae

Depositor

Tineke Lenstra

Publication

Brouwer et al Nat Struct Mol Biol. 2023 May;30(5):692-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PCP-NLS-GFPEnvy

Expression

Mammalian

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKar2-msGFP-HDEL

Plasmid

#117668

Purpose

Localisation of Endoplasmic Reticulum

Depositor

Pau Ferrer, Benjamin Glick

Publication

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pKar2-msGFP-HDEL

Expression

Yeast

Mutation

Contains the Kar2 promoter and signal sequence fr…

Promoter

pKar2

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pRS41K-mScarlet-I-mNeonGreen (pKBJ010)

Plasmid

#173461

Purpose

C-SWAT donor for Seamless tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Publication

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-I-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-RFP-kanMX6

Plasmid

#105160

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Publication

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-RFP-kanMX6

Tags

GBP-RFP

Expression

Bacterial and Yeast

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pHIS3p:mEos2-Tub1+3'UTR::HPH

Plasmid

#50634

Depositor

Wei-Lih Lee

Publication

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mEos2-Tub1

Use

Yeast expression

Tags

mEos2

Promoter

HIS3p

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pKT0232

Plasmid

#8727

Depositor

Kurt Thorn

Publication

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP

Tags

13x Myc and Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pEasyG1_hph

Plasmid

#184912

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Publication

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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