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pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-P90C

Plasmid

#114630

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 49 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 49 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P90C

Plasmid

#114610

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 15 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P70C

Plasmid

#114609

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P70C; 15 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P90C

Plasmid

#114620

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P90C; 37 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P80C

Plasmid

#114619

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P80C cysteine mutations and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, P80C; 37 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-A60C

Plasmid

#114618

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 37 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 37 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-A60C

Plasmid

#114608

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 15 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

A2C, A60C; 15 polyQ repeat

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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Guzit

Bacterial Strain

#196902

Purpose

Guzit is a RNase HI(rnhA) His-tagged E. coli strain made for a cell-free expression system.

Depositor

Kate Adamala

Article

Sato et al Biotechnol Bioeng. 2023 Jul;120(7):1986-

Bacterial Resistance

Kanamycin

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only
pLN472 (FuGW-G8p-STE-1Pe)

Plasmid

#105190

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of STE transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-STE-1Pe

Use

Lentiviral and Synthetic Biology

Tags

HA tag

Expression

Mammalian

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLN429 (FuGW-G8p-mKate2)

Plasmid

#105185

Purpose

Module 3 - GAL4 synthetic promoter ( with eight binding sites) drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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R620-M05-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 K417N/E484K/N501Y

Plasmid

#170191

Purpose

Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Mutation

K417N, E484K, N501Y

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLN193 (SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe))

Plasmid

#105210

Purpose

Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 3 intron - see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe)

Use

Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351

Plasmid

#170196

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Mutation

L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

Promoter

CMV51

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLN381 (FuGW-S(NFY)p-mKate2)

Plasmid

#105179

Purpose

synthetic promoter NFY drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(NFY)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLN592 (FuGW-S(USF1)p-mKate2)

Plasmid

#105204

Purpose

synthetic promoter USF1 drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(USF1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLN593 (FuGW-S(MAFK)p-mKate2)

Plasmid

#105205

Purpose

synthetic promoter MAFK drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(MAFK)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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CoV-NSP3-c001

Plasmid

#173082

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

rep

Tags

mCerulean

Expression

Bacterial

Mutation

E1000-K1173, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pPD1028 ZF43x6-Compact(SV40Min)_EYFP

Plasmid

#138912

Purpose

EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a SV40 minimal promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact(SV40min)

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x6-Compact(SV40min)

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHIE053 ZF43x6-C mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138734

Purpose

Integration Vector, assembled with pHIE045 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLN410 (FuGW-S(E2F1)p-ECFP sponge control)

Plasmid

#105182

Purpose

Module 2 control - synthetic promoter E2F1 drives ECFP only (no miR1 binding sites)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(E2F1)p-ECFP (Cerulean) sponge control

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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