We narrowed to 13,695 results for: crispr cas9

1. SECURE miniABEmax-K20A/R21A (pJUL1774)

   Plasmid

   #131312

   Purpose

   CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM6-G6-vioABE

   Plasmid

   #73438

   Purpose

   Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   PG6-vioABE
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   PG6 (orthogonal T7-lac variant)

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. hA3A-BE3 (pRZ215)

   Plasmid

   #131314

   Purpose

   CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. hAID-BE3 (pRZ352)

   Plasmid

   #131316

   Purpose

   CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. S2 1058 ABEMax

   Plasmid

   #135356

   Purpose

   Expression of intradomain insertion of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at position 1058 of nSpyCas9

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-ABEMax aa 1058
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. S2 1058 V82G ABEMax

   Plasmid

   #135361

   Purpose

   Expression of intradomain insertion of miniABEMax (evolved Tada monomer with V82G) at position 1058 of nSpyCas9

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   1058 nSpyCas9 V82G ABEMax
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   (V82G)

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AIDx-ID-202

   Plasmid

   #135344

   Purpose

   Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 202 of nSpyCas9 used for base editing

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-hAIDx-ID-202
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgRNA_expression_vector

   Plasmid

   #210212

   Purpose

   An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

   Depositor

   Albert Jeltsch

   Article

   Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

   Type

   Empty backbone

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEPOR2KN0040

   Plasmid

   #117607

   Purpose

   Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
   

   Expression

   Plant

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEPOR2KN0063

    Plasmid

    #117630

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_pEPOR1CB0089(pEPOR1CB0089)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Expression

    Plant

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEPOR2KN0062

    Plasmid

    #117629

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113) and sgRNA_UUAT1 {AT5G04160}(pEPOR1CB0087)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
    

    Expression

    Plant

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEPOR2KN0061

    Plasmid

    #117628

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_UUAT1 {AT5G04160}(pEPOR1CB0087)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
    

    Expression

    Plant

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEPOR2KN0057

    Plasmid

    #117624

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtCPK34 {AT5G19360}(pEPOR1CB0084)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Expression

    Plant

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEPOR2KN0053

    Plasmid

    #117620

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Expression

    Plant

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEPOR2KN0032

    Plasmid

    #117604

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0027)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Expression

    Plant

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEPOR2KN0017

    Plasmid

    #117589

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-KA(pEPOR1CB0009) and sgRNA_NbPDS(pEPOR1CB0114)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-KA(pEPOR1CB0009) ] +[AtU6-26:sgRNA]
    

    Expression

    Plant

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEPOR2KN0012

    Plasmid

    #117584

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-DE(pEPOR1CB0008) and sgRNA_NbPDS(pEPOR1CB0114)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-DE(pEPOR1CB0008) ] +[AtU6-26:sgRNA]
    

    Expression

    Plant

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEPOR2KN0004

    Plasmid

    #117579

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0023)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Expression

    Plant

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEPOR2KN0003

    Plasmid

    #117578

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0022)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Expression

    Plant

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEPOR2KN0001

    Plasmid

    #117576

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0114)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    

    Expression

    Plant

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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