We narrowed to 30,801 results for: grna

1. AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI

   Plasmid

   #189925

   Purpose

   AAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   SauriABE8e
   

   Use

   AAV

   Mutation

   SauriCas9 D15A

   Promoter

   EFS

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

   Plasmid

   #138460

   Purpose

   lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   MCP-KRAB-IRES-zsGreen1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKL038 Lenti U6 dCas12a gRNA Puro mCherry

   Plasmid

   #195546

   Purpose

   dCas12a gRNA expression backbone

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti U6- empty cassette_Direct repeat_puro_mcherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-sgRNA EF1Alpha-puro-T2A-BFP

   Plasmid

   #60955

   Purpose

   Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti sgRNA(MS2)_puro optimized backbone

   Plasmid

   #73797

   Purpose

   optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI

   Plasmid

   #189923

   Purpose

   AAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   SaABE8e V106W
   

   Use

   AAV

   Mutation

   nSaCas9 KKH, TadA ABE8e V106W

   Promoter

   EFS

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1

   Plasmid

   #138461

   Purpose

   lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   MCP-VP64-IRES-zsGreen1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Pzac2.1 U6-control sgRNA1, 2, 3_gfaABC1D mcherry SV40

   Plasmid

   #179120

   Purpose

   Expresses control sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

   Depositor

   Baljit Khakh

   Article

   Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

   Insert

   control sgRNA
   

   Use

   AAV

   Promoter

   U6

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657

   Plasmid

   #212962

   Purpose

   Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

   Depositor

   Roland Rad

   Article

   Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

   Insert

   DR1_CasRx_non-targeting spacer
   

   Use

   CRISPR and Lentiviral

   Tags

   TagRFP657

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

    Plasmid

    #212963

    Purpose

    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

    Depositor

    Roland Rad

    Article

    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

    Insert

    DR1_CasRx_GFP targeting spacer
    

    Use

    CRISPR and Lentiviral

    Tags

    TagRFP657

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI

    Plasmid

    #189922

    Purpose

    AAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SaABE8e
    

    Use

    AAV

    Mutation

    SaCas9 D10A

    Promoter

    EFS

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-puro U6 sgRNA BfuAI large stuffer

    Plasmid

    #52628

    Purpose

    lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency

    Depositor

    Scot Wolfe

    Insert

    Kanamycin cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXR004: CasRx pre-gRNA cloning backbone

    Plasmid

    #109054

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid

    #85452

    Purpose

    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

    Depositor

    Aviv Regev

    Article

    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

    Insert

    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid

    #201916

    Purpose

    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Expression

    Mammalian

    Promoter

    CAG and Human U6

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX552-hsyn DIO GCaMP8f(with gRNA scaffold)

    Plasmid

    #199580

    Purpose

    Expresses GCaMP8f in a Cre-dependent manner

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    His-DIO GCaMP8f
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    hsyn

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid

    #201917

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Expression

    Mammalian

    Promoter

    CAG and Human U6

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

    Plasmid

    #220493

    Purpose

    To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

    Depositor

    Sébastien Britton

    Article

    Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

    Insert

    sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

    Plasmid

    #107055

    Purpose

    Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. B52 (empty plasmid backbone to express 2 sgRNAs)

    Plasmid

    #100708

    Purpose

    Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoters

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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