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pMaCTag-P34

Plasmid

#120030

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xFLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-HA-COPS6

Plasmid

#22542

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

COPS6 (COPS6 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mTurquoise2

Plasmid

#99113

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mTurquoise2

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_hU6

Plasmid

#102840

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_hU6promoter

Promoter

hU6

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCLX-CHOP-dGFP

Plasmid

#71299

Purpose

Lentiviral plasmid to express destabilized GFP under the control of the ATF-responsive CHOP promoter

Depositor

Patrick Salmon

Article

Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

Insert

dGFP

Use

Lentiviral

Promoter

CHOP (ATF-responsive)

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pHes1-DN-GFP (CC#110)

Plasmid

#15134

Depositor

Connie Cepko

Article

Furukawa et al Neuron. 2000 May . 26(2):383-94.

Insert

Hes1 (Hes1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

E43A, K44A, R47A

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
pRDA_888

Plasmid

#201165

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLS

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EFS

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET-X(4130)B1

Plasmid

#168018

Purpose

Expresses X(4130)B1-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

X(4130)B1-6xHis

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P28

Plasmid

#120039

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xHA

Use

Pcr template

Tags

3xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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tph-1(short)::rpl-22-HA

Plasmid

#122203

Purpose

C. elegans tagged ribosome expression plasmid

Depositor

Steven Flavell

Article

Rhoades et al Cell. 2019 Jan 10;176(1-2):85-97.e14. do

Insert

tph-1(short)::rpl-22-HA

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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phMYT1L-N106

Plasmid

#31782

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

myelin transcription factor 1-like (MYT1L Human)

Use

Lentiviral

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-TVA-oG-mCherry

Plasmid

#166602

Purpose

Encodes Cre-dependent TVA-oG-mCherry under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

Insert

TVA-oG-mCherry

Use

AAV

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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myc-GABAAR Gamma2

Plasmid

#127619

Purpose

N-terminal myc-tagged Gamma2 subunit of GABAAR

Depositor

Josef Kittler

Article

Arancibia-Carcamo et al Proc Natl Acad Sci U S A. 2009 Oct 13;10

Insert

GABA-A receptor subunit gamma-2

Tags

myc

Expression

Mammalian

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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APT3-Ykt6

Plasmid

#168003

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

Ykt6(L161-L177)-T3'PAP

Use

Synthetic Biology

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCALNL-tdTomato

Plasmid

#125573

Purpose

Cre-dependent expression of tdTomato in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

tdTomato

Use

Cre/Lox

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-tdKatushka2-WPRE

Plasmid

#193337

Purpose

Expresses tdKatushka2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

tdKatushka2

Expression

Mammalian

Promoter

TRE

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCtnnd2.1-GFP

Plasmid

#209095

Purpose

GFP expressing shRNA targeting Ctnnd2 N-terminus

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCtnnd2.1

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-FAM134C-GFP

Plasmid

#201927

Purpose

For lentiviral transduction of FAM134C-GFP

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

FAM134C (RETREG3 Human)

Use

Lentiviral

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P29

Plasmid

#120040

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Strep-II

Use

Pcr template

Tags

Strep-II

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_h7SK

Plasmid

#102842

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_h7SKpromoter

Use

As pcr template

Promoter

h7SK

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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