We narrowed to 16,198 results for: grna

1. pCas9cur

   Plasmid

   #71538

   Purpose

   Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

   Depositor

   Tao Chen

   Article

   Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

   Inserts

   Cas9
   Plasmid curing system
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAT15516_BEAR-GFP-2in1

   Plasmid

   #174082

   Purpose

   BEAR-GFP plasmid with a gRNA that targets its own plasmid

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   EGFP split with an intron between amino acids 95-96
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Intron-Tagging-pX330-Cas9-Blast

   Plasmid

   #159741

   Purpose

   Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   donor plasmid targeting sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

   Plasmid

   #216421

   Purpose

   Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.

   Depositor

   Ellen Bushell

   Article

   Jonsdottir et al bioRxiv 2024.04.20.590404

   Type

   Empty backbone

   Use

   CRISPR; Expression in plasmodium berghei

   Tags

   3xmyc

   Expression

   Bacterial

   Available Since

   April 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPbU6_hdhfr/yfcu

   Plasmid

   #216422

   Purpose

   Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.

   Depositor

   Ellen Bushell

   Article

   Jonsdottir et al bioRxiv 2024.04.20.590404

   Type

   Empty backbone

   Use

   CRISPR; Expression in plasmodium berghei

   Expression

   Bacterial

   Available Since

   April 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQAT59

   Plasmid

   #223431

   Purpose

   T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGC-Lb12-P1Bb

   Plasmid

   #231387

   Purpose

   For crRNA cloning with LbCas12a vectors for genome editing in Arabidopsis

   Depositor

   Qi-Jun Chen

   Article

   Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

   Insert

   U6-29
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXPR_502_sgCD4

   Plasmid

   #158704

   Purpose

   CD4 CRISPRa control

   Depositor

   David Root, John Doench

   Article

   Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

   Insert

   CD4 (CD4 Human)
   

   Use

   Lentiviral

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEB-p53shRNA

   Plasmid

   #28222

   Depositor

   Linzhao Cheng

   Article

   Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

   Insert

   p53 shRNA
   

   Expression

   Mammalian

   Available Since

   March 25, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pYPQ131-tRNA2.0

    Plasmid

    #158393

    Purpose

    Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Without promoter

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC CBA-SpCas9.EF1a-BFP.sgLMNA

    Plasmid

    #98971

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNA and spCas9
    

    Expression

    Mammalian

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJY-RpABE

    Plasmid

    #112872

    Purpose

    binary vector for ABE expression in Arabidopsis

    Depositor

    Jin-Soo Kim, Jae-Young Yun

    Article

    Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

    Inserts

    RPS5A promoter
    ABE7.10
    U6-sgRNA cassette
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiCRISPR_zeo_sgNT135

    Plasmid

    #183181

    Purpose

    pLentiCRISPR that is selectable with zeocin with a non-targeting sgRNA

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    NT135
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQAT60

    Plasmid

    #223432

    Purpose

    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQAT58

    Plasmid

    #223430

    Purpose

    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYPQAT61

    Plasmid

    #223433

    Purpose

    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-dSpCas9-Act3.0-ZmUbi-gRNA scaffold 2.0-tRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJE53

    Plasmid

    #194153

    Purpose

    sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.

    Depositor

    Edward Geisinger

    Article

    Bai et al J Bacteriol. 2021 May 20;203(12):e005652

    Insert

    sgRNA with mrfp control guide sequence
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCRISPR-RFP657

    Plasmid

    #75162

    Purpose

    Derived from LentiCRISPR v1 but expresses RFP657 instead of puromycin resistance

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    RFP657

    Expression

    Mammalian

    Available Since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-Cetn1/1

    Plasmid

    #50718

    Purpose

    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Inserts

    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh and hU6

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. BII-Sh-FUCCI

    Plasmid

    #133363

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Clover-Geminin-IRES-mKO2-Cdt1
    

    Tags

    HA-tagged Clover-Geminin

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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