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pCMV4-HA-p31(comet)

Plasmid

#221697

Purpose

Mammalian expression of HA-tagged human p31(comet)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2BP1

Expression

Mammalian

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV4-HA-MAD2

Plasmid

#221696

Purpose

Mammalian expression of HA-tagged human MAD2

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2

Expression

Mammalian

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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His-GST-ATG13 (363-517)

Plasmid

#222823

Purpose

Bacteria expression of His-GST -ATG13 C-terminal

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

ATG13 (ATG13 Human)

Tags

6His-GST

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-As-NF-kB2

Plasmid

#180201

Purpose

Expresses FLAG-tagged Acanthoeca spectabilis NF-kB2 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB2

Tags

FLAG-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-502

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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H2B-GFP-INCENP∆cen

Plasmid

#184044

Purpose

Expresses H2B-GFP fused to INCENP∆cen

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

INCENP (INCENP Human)

Tags

GFP and H2B

Expression

Mammalian

Mutation

Truncated to 1-862

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Plasmid

#217509

Purpose

KD Recombinase dependent conditional 1X ALFA cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Tags

ALFA tag

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pJT309 RapInducible1-ActivatorCloneSite-Citrine

Plasmid

#187321

Purpose

Rapamycin inducible genetic circuit with cloning site for activator domains

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Type

Empty backbone

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJT310 AAV-BFPdonor

Plasmid

#202057

Purpose

AAV HDR donor

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-BFPdonor

Use

AAV; Aav packaging plasmid

Expression

Mammalian

Mutation

ITR deletion

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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OptoProfilin.S138E

Plasmid

#208287

Purpose

Expresses Cry2(1-531)-mCherry-Profilin.S138E

Depositor

Robert Hughes

Article

Mann et al Chembiochem. 2024 May 2;25(9):e202400007

Insert

Cry2 - mCherry - Profilin.S138E (Cry2, PRF1 Mustard Weed, Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Ser 138 (sometimes numbered 137) mutated to Glu

Promoter

CMV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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OptoProfilin.S138A

Plasmid

#208288

Purpose

Expresses Cry2(1-531)-mCherry-Profilin.S138A

Depositor

Robert Hughes

Article

Mann et al Chembiochem. 2024 May 2;25(9):e202400007

Insert

Cry2 - mCherry - Profilin (Cry2, PRF1 Mustard Weed, Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Ser 138 (sometimes numbered 137) mutated to Ala

Promoter

CMV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST-v2-SF14P

Plasmid

#209448

Purpose

improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoter

Depositor

Tilmann Weber

Article

Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

Insert

Codon optimized APOBEC1-nCas9-UGI fusion protein

Use

CRISPR and Synthetic Biology

Promoter

PtipA; sgRNA expression from SF14P promoter

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST-v2-kasOP*

Plasmid

#209447

Purpose

improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoter

Depositor

Tilmann Weber

Article

Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

Insert

Codon optimized APOBEC1-nCas9-UGI fusion protein

Use

CRISPR and Synthetic Biology

Promoter

PtipA; sgRNA expression from kasOP* promoter

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pDB2-hPER1-Halo-CD4-bla

Plasmid

#189982

Purpose

Donor Vector to integrate HaloTag C-terminal into the human PER1 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

HaloTag

Use

CRISPR

Tags

3xFlag

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDB2-hCRY2-mScarlet-I-CD4-bla

Plasmid

#189984

Purpose

Donor Vector to integrate mScarlet-I C-terminal into the human CRY2 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

mScarlet-I

Use

CRISPR

Tags

3xFlag

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDB2-hCRY2-Luciferase-CD4-bla

Plasmid

#189985

Purpose

Donor Vector to integrate firefly Luciferase C-terminal into the human CRY2 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Luciferase

Use

CRISPR

Tags

3xFlag

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer20-iCreb5(HA)

Plasmid

#195023

Purpose

HA-tagged Creb5 in pInducer20 lentivirus destination vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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GAL4-Creb5 (1- 128)

Plasmid

#195031

Purpose

GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa)

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa) (CREB5 Bovine)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(G137I)

Plasmid

#133272

Purpose

X. laevis expression vector. It will generate the mouse TREK-1 channel

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Use

Oocyte expression

Mutation

G137I

Promoter

T7

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(RUNX3(13))-PGKpuro2ABFP-W

Plasmid

#208552

Purpose

Lentiviral vector expressing gRNA targeting human RUNX3

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RUNX3(13) (RUNX3 Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(RUNX3(12))-PGKpuro2ABFP-W

Plasmid

#208551

Purpose

Lentiviral vector expressing gRNA targeting human RUNX3

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RUNX3(12) (RUNX3 Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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