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pET28a_6HT-TBP6.7(R52A)

Plasmid

#112729

Purpose

52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R52A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R52A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(delta C-term)

Plasmid

#112731

Purpose

This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(delta C-term)

Tags

6His-TEV

Expression

Bacterial

Mutation

delta C-term

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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[pSK480] pLJC5 Flag-Depdc5(A)

Plasmid

#109338

Purpose

Lenti-viral expression of Flag-tagged Depdc5 mutant A

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Mutation

DIYGD(185-189)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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[pSK481] pLJC5 Flag-Depdc5(B)

Plasmid

#109339

Purpose

Lenti-viral expression of Flag-tagged Depdc5 mutant B

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Mutation

EQPLH(371-375)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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[pSK479] pLJC5 Flag-Depdc5(C)

Plasmid

#109340

Purpose

Lenti-viral expression of Flag-tagged Depdc5 mutant C

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Mutation

FTPRI(419-423)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-dCEN-Incenp

Plasmid

#108479

Purpose

expression of vsv-dCEN-INCENP

Depositor

Susanne Lens

Article

Vader et al Mol Biol Cell. 2007 Nov;18(11):4553-64.

Insert

dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box removed

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_FNBP1-1/1

Plasmid

#91552

Purpose

Protein expression and purification of human SH3 domain construct FNBP1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FNBP1-1/1 (FNBP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ABL2-1/1

Plasmid

#91533

Purpose

Protein expression and purification of human SH3 domain construct ABL2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ABL2-1/1 (ABL2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_CACNB2-2/2

Plasmid

#91507

Purpose

Protein expression and purification of human SH3 domain construct CACNB2-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CACNB2-2/2 (CACNB2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_CD2AP-3/3

Plasmid

#91508

Purpose

Protein expression and purification of human SH3 domain construct CD2AP-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CD2AP-3/3 (CD2AP Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DLG5-1/1

Plasmid

#91522

Purpose

Protein expression and purification of human SH3 domain construct DLG5-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DLG5-1/1 (DLG5 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_CASKIN1-1/1

Plasmid

#91523

Purpose

Protein expression and purification of human SH3 domain construct CASKIN1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CASKIN1-1/1 (CASKIN1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGAP26-1/1

Plasmid

#91484

Purpose

Protein expression and purification of human SH3 domain construct ARHGAP26-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGAP26-1/1 (ARHGAP26 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ITSN2-4/5

Plasmid

#91496

Purpose

Protein expression and purification of human SH3 domain construct ITSN2-4/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN2-4/5 (ITSN2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ITSN2-1/5

Plasmid

#91497

Purpose

Protein expression and purification of human SH3 domain construct ITSN2-1/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN2-1/5 (ITSN2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_EPS8L1-1/1-IS1/2

Plasmid

#91465

Purpose

Protein expression and purification of human SH3 domain construct EPS8L1-1/1-IS1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

EPS8L1-1/1-IS1/2 (EPS8L1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ITSN2-3/5

Plasmid

#91470

Purpose

Protein expression and purification of human SH3 domain construct ITSN2-3/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN2-3/5 (ITSN2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_FCHSD2-1/2

Plasmid

#91444

Purpose

Protein expression and purification of human SH3 domain construct FCHSD2-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FCHSD2-1/2 (FCHSD2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DLG1-1/1

Plasmid

#91448

Purpose

Protein expression and purification of human SH3 domain construct DLG1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DLG1-1/1 (DLG1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_FCHSD1-1/2

Plasmid

#91449

Purpose

Protein expression and purification of human SH3 domain construct FCHSD1-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FCHSD1-1/2 (FCHSD1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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