Addgene

We narrowed to 26,848 results for: Tat

Showing: 1141 - 1160 of 26848 results

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pET-Cdc12-Cdc11

Plasmid

#131162

Purpose

Expression of Septin rods

Depositor

Nils Johnsson

Article

Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

Insert

6his-Cdc12 + Cdc11

Use

Bicistronic

Tags

Cdc11: S-Tag and Cdc12: 6his

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAc-Cdc3-Cdc10

Plasmid

#131160

Purpose

Expression of Septin rods

Depositor

Nils Johnsson

Article

Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

Insert

Cdc3 + Cdc10

Use

Bicistronic

Tags

Cdc10: S Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pXCX-sGFAP-LMO-IRES-tdTomato

Plasmid

#178312

Purpose

Express the bacterial lactate 2-monooxygenase (LMO) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

Lactate 2-monooxygenase

Use

Adenoviral

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

sGFAP

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-BssgRNA

Plasmid

#121957

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

BsCas12b single chimeric gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSL6 SMR1747

Plasmid

#28029

Depositor

Susan Rosenberg

Article

Harris et al Genes Dev. 1997 Sep 15. 11(18):2426-37.

Insert

MutS (mutS E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 1, 2011

Availability

Academic Institutions and Nonprofits only
pSL1315

Plasmid

#129527

Purpose

pDEF-SpCas9, Best (-458) Vector for CRISPRi of PyALBA4, PbEF1a promoter, 1st Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

dSpCas9

Use

Plasmodium, rodent-infectious species

Tags

NLS, 1xHA

Expression

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pESC-LEU2-RAD30cmyc

Plasmid

#60821

Purpose

to overexpress yeast polymerase eta in yeast

Depositor

Thomas Kunkel, Youri Pavlov

Article

Pavlov et al Mutat Res. 2001 Jul 1;478(1-2):129-39.

Insert

RAD30 (RAD30 Budding Yeast)

Use

Tags

c-myc

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
SHLD3 KO sgRNA #1

Plasmid

#207105

Purpose

pX330 based plasmid for expression of Cas9 and the CGCTATCAAGATTTATACCT sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CGCTATCAAGATTTATACCT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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IAA17-4flag-HIS5

Plasmid

#207015

Purpose

For ORF tagging with IAA17-4Flag. IAA17 is an auxin-inducible degron (AID).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-HIS5

Plasmid

#207014

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker HIS5 (Schizosaccharomyces Pombe)

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-MET15

Plasmid

#207013

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker MET15 (Saccharomyces cerevisiae)

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-URA3

Plasmid

#207012

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker URA3 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_IHFdist_rm

Plasmid

#162318

Purpose

P. aeruginosa PA14 CRISPR2 locus, with the IHFdistal binding site removed

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

IHF distal site is removed

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pXCX-sGFAP-DLDH-IRES-tdTomato

Plasmid

#178311

Purpose

Express the bacterial D-lactate dehydrogenase (DLDH) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

D-lactate dehydrogenase

Use

Adenoviral

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

sGFAP

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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OA-1050Z4 (yellow)

Plasmid

#132425

Purpose

express arrays of gRNA targeting Yellow under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

yellow gRNA array

Use

Tags

Expression

Insect

Mutation

Promoter

U6-3

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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Ad-mMKP3 S159A S197A-Flag

Plasmid

#131579

Purpose

Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells

Depositor

Haiyan Xu

Insert

Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)

Use

Adenoviral

Tags

Flag

Expression

Mutation

changed Serine 159 to Alanine and Serine 197 to A…

Promoter

CMV

Available since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAc-Cdc3-Cdc10-SNAP

Plasmid

#131161

Purpose

Expression of Septin rods

Depositor

Nils Johnsson

Article

Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

Insert

Cdc3 + Cdc10-SNAP

Use

Bicistronic

Tags

Cdc10: SNAP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCML 220

Plasmid

#98588

Purpose

pCYFP. Expresses CYFP control fusion in E. coli for TriFC assay (kanR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLacO

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSL5 SMR1713

Plasmid

#28028

Depositor

Susan Rosenberg

Article

Harris et al Genes Dev. 1997 Sep 15. 11(18):2426-37.

Insert

MutL (mutL E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 1, 2011

Availability

Academic Institutions and Nonprofits only
rApoI

Bacterial Strain

#156459

Purpose

An E. coli strain that expresses rat APOBEC1. Used as a control strain for mutagenesis experiments with MutaT7.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Bacterial Resistance

Streptomycin

Species

Rattus norvegicus

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only