We narrowed to 3,057 results for: try
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Plasmid#84989PurposeType 7, BxbI recognition siteDepositorInsertBxbI recognition site
UseSynthetic BiologyExpressionYeastMutationBsaI site removed (1711 t to a)PromoterNonAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE-WT-N4-R5-YRNRV
Plasmid#63625PurposeN4-R5-YRNRV RNAP mutantDepositorInsertN4-R5-YRNRV RNAP
MutationN671Y, L747I, N748D, L749C, M750V, F751I, Q754T, …PromoterT5/LacAvailable SinceJune 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
EmptyTranscriptionalReporter
Plasmid#62453PurposeMXS_chaining vector with 2 cassettesDepositorInsertsCMVmin::NLS-Citrine-bGHpA
CMV::NLS-Cherry-bGHpA
UseSynthetic BiologyAvailable SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MXS_CMV::tTA2-bGHpA
Plasmid#62447PurposeMXS_chaining vector with CMV::tTA2-bGHpADepositorInsertcassette containing the tetracycline transactivator with CMV promoter
UseSynthetic BiologyAvailable SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFSW GAP43-jRGECO1a P2A Syp-Halo
Plasmid#185565PurposeExpression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequenceDepositorInsertGAP43-jRGECO1a P2A Syp-Halo
UseLentiviralTagsHaloTagAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pATZ-HA-GFP11
Plasmid#177720PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
TagsGFP11 and HAExpressionBacterial and MammalianMutationE342K (ATZ mutant)Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW_TOPO_Cdk12_Untagged
Plasmid#127176PurposeUntagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vectorDepositorInsertMouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
UseEntry vector with transgene for gateway cloningTagsNonePromoterNoneAvailable SinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPTK009-3a-αAmylase-αMFΔ
Plasmid#84968PurposeType 3a, α-Amylase followed by αMFΔ - secretion signalDepositorInsertα-Amylase followed by MFΔ secretion tag
UseSynthetic BiologyExpressionYeastPromoterNonAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK018-3b-RFP_Sec
Plasmid#84977PurposeType 3b, Red fluorescent proteinDepositorInsertRed fluorescent protein
UseSynthetic BiologyExpressionYeastMutationBsaI site removed (1677 c to g and 1794 a to g)PromoterNonAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
phpBCL2-FL(pA)
Plasmid#42593DepositorInserthairpin - BCL2 5'UTR - firefly luciferase
UseLuciferaseExpressionMammalianPromoterCMVAvailable SinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M2-TLR2-FW/AA
Plasmid#12293DepositorInsertTLR2 (Tlr2 Mouse)
TagsFLAG (FLAG-M2)ExpressionMammalianMutationM82I, H202Y, F774A and W775A; signal peptide (aa1…Available SinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)
Plasmid#139445PurposeLentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoterDepositorInsertFLAG-tag E2-crimson
UseLentiviralTagsFLAGExpressionMammalianPromoterSFFVAvailable SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
phpRL-BCL2-FL-pA
Plasmid#42595DepositorInserthairpin - renilla luciferase - BCL2 5'UTR - firefly luciferase
UseLuciferaseExpressionMammalianPromoterCMVAvailable SinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHJ41
Plasmid#48360PurposeTargets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Blasticidin/Gancyclovir selectableDepositorInsertPartial beta-TUB followed by 5' ALD UTR-loxP-SAS-BSD-Ty1-TK-loxP-3' ALD UTR
UseCre/Lox; Knockout in t. bruceiAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAcUW51-gE2
Plasmid#11621DepositorInsertHSV-1 glycoprotein E
TagsHisExpressionInsectMutationdeleted amino acids 391-550Available SinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
prDNA spacer-LacOx50-20xMS2-Hyg
Plasmid#210219PurposeIntegrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.DepositorInserts50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865
Available SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF-L3-NF-L1-dMb2Cas12a
Plasmid#177686PurposeGateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editingDepositorInsert2xFokI-dMb2Cas12a
UseCRISPR; Gateway compatible 2xfoki-dmb2cas12a entr…TagsNLSExpressionPlantAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAP03-act1p
Plasmid#120233PurposeCarries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.DepositorInsertpromoter cassette containing act1p promoter and apramycin resistance gene
UseSynthetic BiologyMutationaac(3)IV (apramycin resistance gene)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo-CTRLg1
Plasmid#139450PurposeLentiviral vector with non-targeting gRNA and neomycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: neoR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo-CTRLg2
Plasmid#139451PurposeLentiviral vector with non-targeting gRNA and neomycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: neoR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only