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pKLV2-U6gRNA5(AAVS1)-PGKpuro2ABFP-W

Plasmid

#200459

Purpose

Lentiviral vector expressing gRNA targeting human AAVS1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

AAVS1 (AAVS1 Human)

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Control(1))-PGKpuro2AmCherry-W

Plasmid

#210612

Purpose

Lentiviral vector expressing gRNA targeting a control region at the CXCR4 locus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

Control(1)

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(RIC8A(16))-PGKpuro2ABFP-W

Plasmid

#200491

Purpose

Lentiviral vector expressing gRNA targeting human RIC8A

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RIC8A(16) (RIC8A Human)

Use

Lentiviral

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(RIC8A(15))-PGKpuro2ABFP-W

Plasmid

#200490

Purpose

Lentiviral vector expressing gRNA targeting human RIC8A

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RIC8A(15) (RIC8A Human)

Use

Lentiviral

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Control(2))-PGKpuro2AmCherry-W

Plasmid

#210613

Purpose

Lentiviral vector expressing gRNA targeting a control region at the CXCR4 locus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

Control(2)

Use

Lentiviral

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-Δ220-234-Flag

Plasmid

#210341

Purpose

Expresses SARS-CoV-2 NSP4-Δ220-234 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Tags

Flag

Expression

Mammalian

Mutation

Δ220-234

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-H120N/F121L-Flag

Plasmid

#210340

Purpose

Expresses SARS-CoV-2 NSP4-H120N/F121L fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Tags

Flag

Expression

Mammalian

Mutation

H120N/F121L

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-FR-2Strep

Plasmid

#210316

Purpose

Expresses SARS-CoV-2 NSP4 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Tags

2Strep

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-mCherry

Plasmid

#210319

Purpose

Expresses SARS-CoV-2 NSP4 fused with mCherry in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Tags

mCherry

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP10-2Strep

Plasmid

#210357

Purpose

Expresses FKBP10 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

FKBP10 (FKBP10 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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DNAJC11-2Strep

Plasmid

#210354

Purpose

Expresses DNAJC11 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

DNAJC11 (DNAJC11 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-FIP200-L324D/R543D/R548D-mCherry-Hygro

Plasmid

#210856

Purpose

Lentiviral expression vector encoding FIP200 mutant-mCherry

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

FIP200 (RB1CC1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

L324D/R543D/R548D

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG13 (363-450)

Plasmid

#210849

Purpose

Mammalian expression of ATG13 (363-450) mutant

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

ATG13 (ATG13 Human)

Expression

Mammalian

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-ZNF746-C571A

Plasmid

#207821

Purpose

N-terminal tagged ZNF746-C571A for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-ZNF746-C571A (ZNF746 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Mutation

C571A

Promoter

UBC

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9-ZNF746

Plasmid

#207822

Purpose

Dual expression of Cas9 and sgRNA targeting ZNF746

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

ZNF746 sgRNA (ZNF746 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

EF-1a; U6

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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rUBR5_2383-2442_pGEX6P1

Plasmid

#209791

Purpose

Bacterial expression of UBR5 Mlle domain

Depositor

Kalle Gehring

Article

Lim et al J Biol Chem. 2006 May 19;281(20):14376-8

Insert

Mlle domain of UBR5 (Ubr5 Rat)

Tags

GST

Expression

Bacterial

Mutation

aa 2383-2442 only

Promoter

tac

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti7-3_EF1ap-mRuby2_hU6p_sgSTK4i_#1 (BlasR)

Plasmid

#209776

Purpose

CRISPRi for STK4

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

Nluc, sgSTK4i, mRuby2 (STK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_dCas9-KRAB_sgSTK4i_#1 (PuroR)

Plasmid

#209773

Purpose

CRISPRi for STK4

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

dCas9-KRAB, sgSTK4i (STK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_dCas9-KRAB_sgSTK4i_#2 (PuroR)

Plasmid

#209774

Purpose

CRISPRi for STK4

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

dCas9-KRAB, sgSTK4i (STK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGEMHE-MmCENP-O-GFP

Plasmid

#205178

Purpose

Expresses mouse CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcription

Depositor

Michael Lampson

Article

Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

Insert

CENP-O (Cenpo Mouse)

Use

In vitro transcription

Tags

5 glycines linker followed by GFP

Expression

Mammalian

Mutation

Wild type mouse CENP-O

Promoter

T7

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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