We narrowed to 19,362 results for: gateway

Showing: 11581 - 11600 of 19362 results

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MSCVhyg-HA-FLAG_Ubc9_WT

Plasmid

#164936

Purpose

Expresses FLAG & HA tagged human uman Ubiquitin Conjugating Enzyme 9 (UBC9) wild type cDNA

Depositor

Ji Luo

Publication

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

human UBC9 (UBE2I Human)

Use

Retroviral

Tags

FLAG & HA tags

Promoter

PGK promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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R725-X20-566: His6-MBP-tev-Avi-Hs.LGALS3

Plasmid

#159700

Purpose

E. coli protein expression

Depositor

Dominic Esposito

Publication

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-MBP-tev-Avi-Hs.LGALS3 (LGALS3 Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS011E: Entry vector for: superecliptic pHluorin (mitochondrial)

Plasmid

#163067

Purpose

Entry vector for: superecliptic pHluorin (mitochondrial)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

superecliptic pHluorin

Use

Gateway Cloning

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS017E: Entry vector for: ratiometric pHluorin (cytosolic)

Plasmid

#163069

Purpose

Entry vector for: ratiometric pHluorin (cytosolic)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Gateway Cloning

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS009E: Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

Plasmid

#163062

Purpose

Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

QuasAr2

Use

Gateway Cloning

Tags

mOrange2

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKV NS5

Plasmid

#145348

Purpose

Cell-free/ mammalian expression vector for Zika virus NS5 protein with N-term eGFP

Depositor

Rob Parton

Publication

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS5

Tags

8xHis and eGFP

Expression

Bacterial

Promoter

T7- CMV/CAG

Available since

March 3, 2021

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKV NS2A

Plasmid

#145343

Purpose

Cell-free/mammalian expression vector for Zika virus NS2A protein with N-term eGFP

Depositor

Rob Parton

Publication

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2A

Tags

8xHis and eGFP

Expression

Bacterial

Promoter

T7- CMV/CAG

Available since

Feb. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB701NL3F10H

Plasmid

#141288

Purpose

No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, Tunicamycin R for plant)

Depositor

Andrew Millar

Publication

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Type

Empty backbone

Use

Gateway Cloning and Luciferase

Tags

FLAG (x3), HIS (x10), and NLUC

Expression

Plant

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLVX-Flag-Polß(WT)-IRES-puro

Plasmid

#128653

Purpose

mammalian expression of Flag-Polß(WT) protein with puromycin selection

Depositor

Robert Sobol

Publication

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCFJ2370 - intron GG3 - 250bp

Plasmid

#159879

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2259 - Intron GG2 - 900 bp

Plasmid

#159881

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - 900 bp

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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907H1

Plasmid

#160234

Purpose

FlightN-CymR-VP16-3X-SV40-attB

Depositor

Omar Akbari

Publication

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

p-cymene repressor
Flightin promoter

Tags

VP16

Expression

Insect

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP15

Plasmid

#158366

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP15 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available since

Nov. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLEX307-SARS-CoV-3CL-C145A

Plasmid

#160281

Purpose

Allows for constituitive mammalian expression of the SARS-CoV 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Publication

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C145A

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X09-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D

Plasmid

#159541

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D

Depositor

Dominic Esposito

Publication

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12D

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLX311-SHOC2-V5

Plasmid

#134521

Purpose

Lentiviral plasmid expressing SHOC2 with V5 C-term tag

Depositor

Andrew Aguirre

Publication

Sulahian et al Cell Rep. 2019 Oct 1;29(1):118-134.e8. d

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Silent mutations made at wobble base position at …

Promoter

EF1alpha

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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MAC-TMPRSS2

Plasmid

#158385

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

TMPRSS2 (TMPRSS2 Human)

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 26, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 Orf3b

Plasmid

#158373

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf3b

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 26, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 Orf7b

Plasmid

#158376

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf7b (ORF7b )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP7

Plasmid

#158358

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP7 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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