We narrowed to 27,377 results for: Cas9
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Plasmid#115490PurposeCRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa VP64 Neurog2
Plasmid#99695PurposeExpresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAVDepositorPublicationInsertdCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Synthetic, Mouse)
UseAAV and CRISPRTagsVP64Mutationdead Cas9Available sinceSept. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
BFP Homology Donor
Plasmid#149372PurposePromoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCDDR BFP Homology Donor
ExpressionMammalianAvailable sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCR1265
Plasmid#111094Purposelentiviral cDNA expression vector: plenti-x1-neo-linker-IRES-Thy1.1DepositorTypeEmpty backboneUseLentiviralAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAdSh.U6.gRNAS1
Plasmid#58252PurposeU6 promoter-driven single guide RNA complementary to the human AAVS1 locusDepositorInsertU6.gRNAS1 cassette
UseAdenoviral and CRISPRExpressionMammalianAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN3
Plasmid#180284PurposeSpacer cassette (TU2)DepositorInsertConL1 - Spacer - ConR2
ExpressionBacterialAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
LBJJ382
Plasmid#117518PurposeAlso known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminatorDepositorInsertBpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI
UseCRISPRExpressionPlantPromoterAtU6-26Available sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_Tet-inducible Luciferase reporter
Plasmid#64161PurposePhotoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA1 for Tet-inducible Luciferase reporter
UseCRISPRExpressionMammalianPromoterhuman U6Available sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJA54
Plasmid#60212Purposecleavage near e1350 locus in sqt-1DepositorInsertsqt-1(e1350) gRNA1
UseCRISPRPromoterU6Available sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
reporter-gT2
Plasmid#47321PurposeTF reporter for gRNA-AAVS1_T2 (RNA-guided dTomato expression)DepositorInsertminCMV-dTomato_pA
UseCRISPRAvailable sinceAug. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJAT7
Plasmid#204292PurposeFor inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3XP3-DsRed
UseCRISPRPromoter3XP3Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEN-sacB
Plasmid#196249PurposePlasmid containing the sacB geneDepositorInsertattR2-sacB-attL3
ExpressionBacterialPromotersacB promoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141D
Plasmid#69293PurposeGateway entry vector; single gRNA under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LentiCRISPRv2 RFP670
Plasmid#187646Purposelentiviral vector expressing RFP670 alongside Cas9 and an sgRNA cloning siteDepositorInsertRFP 670
UseLentiviralTagsRFP670 and sgRNA cloning sitePromoterEFS (P2A)Available sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pACRISPR-KM
Plasmid#203587PurposeA sgRNA expression plasmid for genome editing in BurkholderiaDepositorTypeEmpty backboneExpressionBacterialAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19/PLIN2-GFP
Plasmid#202194Purposeknock-in donor vector of GFP to the C-terminus of endogenous human PLIN2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPLIN2 (PLIN2 Human)
UseCloning vectorTagsGFP knock-inPromoterlacAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
PuroR-P2A-DD-TCOF1-donor
Plasmid#60747PurposeDonor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1DepositorInsertTCOF1 (TCOF1 Human)
UseTargeting donorAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
AALN-BE4max
Plasmid#138161PurposeC-to-T base editorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertrAPOBEC1(AALN)-nSpCas9-UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 R33A + K34A + H122L + D124N; with…PromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB8788
Plasmid#126922PurposePlasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI and HMG1DepositorInsertXdCrtI and HMG1
ExpressionYeastAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only