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pGAL4-DBD-HOXD4-AroPLUS_LITE

Plasmid

#215583

Purpose

For overexpression of Gal4-DBD HOXD4 AroPLUS LITE

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXD4 AroPLUS LITE (HOXD4 Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EGR1-AroPATCHY1

Plasmid

#215596

Purpose

For overexpression of Gal4-DBD EGR1 AroPATCHY1

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

EGR1 AroPATCHY1 (EGR1 Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-CEBPa-AroPERFECT_IS10

Plasmid

#215606

Purpose

For overexpression of Gal4-DBD CEBPa AroPERFECT IS10

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa AroPERFECT IS10 (CEBPA Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-CEBPa-AroLITE_A

Plasmid

#215602

Purpose

For overexpression of Gal4-DBD CEBPa AroLITE

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa AroLITE (CEBPA Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_min1-2_4XEboxMutant

Plasmid

#215026

Purpose

Firefly luciferase enhancer reporter plasmid with minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated

Use

Luciferase

Mutation

4X E-box mutations within Coordinator motifs

Promoter

SV40

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pHES7-NLuc-2A-tdTomato

Plasmid

#204348

Purpose

Donor vector to tag endogenous pig HES7 locus

Depositor

Li-Fang Chu

Article

Conrad et al Stem Cell Reports. 2023 Dec 12;18(12):23

Insert

NLuc-2A-tdTomato

Tags

NanoLuc and tdTomato

Expression

Mammalian

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EFNB2-RBD-Fc (D62Q)

Plasmid

#200979

Purpose

Mammalian expression plasmid for soluble EFNB2 (D62Q specificity mutant) fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Tags

IgG1 Fc

Expression

Mammalian

Mutation

Soluble Receptor-Binding Domain; D62Q mutation fo…

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Gata6-3xT7

Plasmid

#200889

Purpose

Inducible lentiviral expression of Gata6

Depositor

Kai Ge

Article

Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

Insert

Gata6 (Gata6 Mouse)

Use

Lentiviral; Doxycycline inducible

Tags

3xT7

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pETDuet-MerTK_KRmutant-PTPN1

Plasmid

#196447

Purpose

Expression of MerTK Kinase Domain in E. coli and surface entropy reduction mutant for crystallization, coexpress PTPN1

Depositor

Byung Il Lee

Article

Bae et al Exp Mol Med. 2022 Sep;54(9):1450-1460. d

Inserts

Tyrosine-Protein Kinase Mer (MERTK Human)
PTPN1 (PTPN1 Human)

Expression

Bacterial

Mutation

K591R, K639R, K702R, K865R

Promoter

T7 promoter

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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kanMX6-ins1

Plasmid

#195038

Purpose

pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tDEG1), allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

KanR

Use

Yeast genomic targeting

Tags

S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCavin1a in pT3TS-Dest

Plasmid

#194294

Purpose

In vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAI

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1a (cavin1a Zebrafish)

Use

In vitro transcription of mrna

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pIFEX-A2aGH

Plasmid

#160542

Purpose

pITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEFEX1-A2aGH

Plasmid

#160543

Purpose

pYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEFEX2-A2aGH

Plasmid

#160544

Purpose

pYES2 derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEFEX3-A2aGH

Plasmid

#160545

Purpose

pYES2 derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCFEX1-A2aGH

Plasmid

#160546

Purpose

pYC2/CT derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCFEX2-A2aGH

Plasmid

#160547

Purpose

pYC2/CT derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCFEX3-A2aGH

Plasmid

#160548

Purpose

pYC2/CT derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pSN687

Plasmid

#177363

Purpose

Expresses human KIF1A(1-393)(P305L) fused with leucine zipper and His tag

Depositor

Shinsuke Niwa

Article

Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

KIF1A (KIF1A Human)

Tags

His tag and Leucine zipper

Expression

Bacterial

Mutation

changed Proline 305 to Leucine

Promoter

T7

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCSmEn2ERT(#117)

Plasmid

#184079

Purpose

cyclofen-inducible chicken EN2 (wt) activation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

6his-myc-chkEn2-ERT2

Use

Synthetic Biology

Tags

6his-Myc

Expression

Mammalian

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

sCMV+SP6

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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