We narrowed to 23,671 results for: CRISPR

1. pGH156_MS2-AID-Hygro

   Plasmid

   #85415

   Purpose

   lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   MS2-AID and Hygromycin resistance
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMJ915v2 + sfGFP

   Plasmid

   #88918

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-LbCpf1-U6_FlipArray

   Plasmid

   #109349

   Purpose

   Lentiviral vector to express Cpf1 from EFS promoter, as well as a Cpf1 FlipArray from U6 promoter.

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Commun. 2018 May 15;9(1):1903. doi:

   Insert

   U6-FlipArray
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-GFP-SFPQ

   Plasmid

   #97084

   Purpose

   Encodes an sgRNA that creates a DSB at the promoter of human SFPQ gene.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA GFP-SFPQ
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. px330 VRER

   Plasmid

   #101729

   Purpose

   Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E, and T1337R mutation…

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. LT0

   Plasmid

   #210551

   Purpose

   Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporter

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   pU6::AAVS1gRNA::LT0::mCherrySSM
   

   Expression

   Plant

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. LT1

   Plasmid

   #210552

   Purpose

   Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporter

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   pU6::AAVS1gRNA::LT1::mCherrySSM
   

   Expression

   Plant

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. LT2

   Plasmid

   #210553

   Purpose

   Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporter

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   pU6::AAVS1gRNA::LT2::mCherrySSM
   

   Expression

   Plant

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pgRNA-non-target

   Plasmid

   #129465

   Purpose

   Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

   Depositor

   Silvia Cardona

   Article

   Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

   Insert

   Non-target/random gRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   BBa_J23119 (SpeI)

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. 1xNLS-pMJ915v2

    Plasmid

    #88915

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFH75

    Plasmid

    #128224

    Purpose

    classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV_miniABEmaxNG(GG)

    Plasmid

    #187062

    Purpose

    miniABEmax(GG)-SpCas9-NG

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    miniABEmax(GG)-SpCas9-NG
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-TREX1 g1 Cas9-T2A-mCherry

    Plasmid

    #164250

    Purpose

    Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgTREX1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRDA_450

    Plasmid

    #179094

    Purpose

    nuclease Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    SpRY
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDYSCKO-ADE1

    Plasmid

    #120264

    Purpose

    pDYSCKO canavanine co-selection vector with a guide targeting the ADE1 locus

    Depositor

    Christian Landry

    Article

    Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

    Insert

    pDYSCKO cassette-ADE1 targeting guide
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    SNR52

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMS15: pTwinStrep-SUMO-AvTnsB

    Plasmid

    #168148

    Purpose

    AvTnsB expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    AvCAST TnsB
    

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMS16: pTwinStrep-SUMO-AvTnsC

    Plasmid

    #168149

    Purpose

    AvTnsC expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    AvCAST TnsC
    

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMS14: pTwinStrep-SUMO-AvTnsA

    Plasmid

    #168147

    Purpose

    AvTnsA expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    AvCAST TnsA
    

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p458 VRER

    Plasmid

    #101728

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. p330 VQR

    Plasmid

    #101730

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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