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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,126 results for: LEA

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Showing: 11681 - 11700 of 12126 results

  3. Myc-Nix-S35D

    Plasmid
    #197563
    Purpose
    Mammalian expression of myc-tagged human Nix with an aspartic acid substitution of serine-35. Based on Myc-Nix (#100795)
    Depositor
    Joseph Gordon
    Article
    Field et al Autophagy. 2025 Jul;21(7):1544-1555. doi
    Insert
    BCL2 interacting protein 3 like (BNIP3L Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Serine 35 changed to aspartic acid
    Promoter
    CMV
    Available Since
    March 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    197563-plasmid-map-sequence-id-386250

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  5. pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

    Plasmid
    #185492
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185492-plasmid-map-sequence-id-363405

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  6. pαH-rS2d

    Plasmid
    #183516
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein S-GSAS-rS2d with (682-685) furin side replaced with GSAS and mutation S383C, D985C
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    rS2d (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    May 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    183516-plasmid-map-sequence-id-362651

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  7. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

    Plasmid
    #179527
    Purpose
    For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 K233A/K234A/K253A/K263A
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    changed lysines 233, 234, 253, and 263 to alanine
    Available Since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179527-plasmid-map-sequence-id-351733

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  8. pPodA11

    Plasmid
    #162843
    Purpose
    Expression protein designed PodA10 from a TEV cleavable IPTG inducible plasmid with ampicillin resistance
    Depositor
    Dianne Newman
    Article
    VanDrisse et al Proc Natl Acad Sci U S A. 2021 Mar 23;11
    Insert
    Protein Designed Pyocyanin demethylase
    Tags
    TEV-cleavable 6x-His tag
    Expression
    Bacterial
    Mutation
    A53N, I73T, A87V, M99V, A129T from WT PodA
    Promoter
    T7
    Available Since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    162843-plasmid-map-sequence-id-343491

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  11. pGBW-m4046500

    Plasmid
    #149063
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;MBP
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149063-plasmid-map-sequence-id-299929

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  12. pGBW-m4046567

    Plasmid
    #148972
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    148972-plasmid-map-sequence-id-299913

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  13. pBB0405-TEV-His12

    Plasmid
    #137040
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0405 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66795.1 (BB_0405 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137040-plasmid-map-sequence-id-275693

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Showing: 11681 - 11700 of 12126 results