We narrowed to 15,989 results for: LEA
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Plasmid#149527PurposeBacterial expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
Tags6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
OSCA1.2-PP-GFP
Plasmid#135390PurposeExpresses OSCA1.2 in mammalian cellsDepositorInsertOSCA1.2 (AT4G22120 Mustard Weed)
TagsEGFP tag connected to the gene via a precision pr…ExpressionMammalianPromoterCMVAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046901
Plasmid#145725PurposeBacterial Expression plasmid for SARS-CoV-2 ORF7a proteinDepositorInsertSARS-CoV-2 ORF7a protein (ORF7a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)
Plasmid#191801PurposeFull length wt AtPCO4 with Nt-His tagDepositorInsertAtPCO4-1
Tags6xHis, TEV cleavage siteExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
CXXC1
Plasmid#32045PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCXXC1 (CXXC1 Human)
TagsHis (with TEV cleavage)ExpressionBacterialMutationCXXC domain (amino acids residues 161-222)Available SinceJan. 3, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMH-HT_CtCHAD
Plasmid#127785PurposeExpresses CtCHAD in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain)Available SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJZC595
Plasmid#62322PurposeExpresses MCP-VP64 and dCas9 in Yeast cellsDepositorInsertsMCP-VP64
dCas9
TagsVP64ExpressionYeastMutationHas nuclease-inactivating mutations in the RuvC1 …PromoterTdh3 and pAdhAvailable SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046764
Plasmid#145673PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;StrepIIExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMEH294 NLS dTomato 1x high affinity MS2 loop
Plasmid#168213PurposeMammalian expression of nuclear-localized dTomato with 1 high affinty MS2 loopDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-alphaCaMKII-E2-Crimson-3XNLS-pA
Plasmid#183803PurposeAAV vector to express nuclear localized E2-Crimson from CaMKIIa promoterDepositorInsertE2-Crimson-NLS
UseAAVTagsNLSAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xFNLDD/pCMV-7.1
Plasmid#48874PurposeExpresses 3xFNLDD in mammalian cellsDepositorInsert3xFNLDD
Tags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianPromoterCMV promoterAvailable SinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
DNMT3B (3QKJ)
Plasmid#32044PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDNMT3B (DNMT3B Human)
TagsHis (with TEV cleavage)ExpressionBacterialMutationPWWP domain only (amino acids 293-442)Available SinceFeb. 22, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
fADL-1e-flag-linker-p3
Plasmid#139441PurposeFor cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.DepositorInsertlinker with pelB-leader, cloning site, flag-tag and p3
ExpressionBacterialAvailable SinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
V1-MESA-45F-M-dCas9
Plasmid#84504PurposeMESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64DepositorInsertV2-MESA-35F-M-dCas9
UseLentiviralTagsFlagExpressionMammalianPromoterCMVAvailable SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBH31(gfcD)
Plasmid#176199PurposeEncodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasmDepositorInsertgfcD
TagspelC leader-6xHisExpressionBacterialPromoterT7Available SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBH31(gfcD-10xHis)
Plasmid#176200PurposeEncodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasmDepositorInsertgfcD
TagspelC leader-10xHisExpressionBacterialPromoterT7Available SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_SsCHAD
Plasmid#127783PurposeExpresses SsCHAD in E. coli cellsDepositorInsertSsCHAD (SULM_RS11045 Sulfolobus solfataricus)
Tags6xHis tag + TEV cleavage siteExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myog'-2A-mCherryNLS-PGK-Purodtk
Plasmid#69547PurposeDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.DepositorInsertsUseMouse TargetingTags2x nuclear localization signal and PVT1-2A "…MutationSilent V205V mutation in myogenin gene to prevent…Available SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330(puro) Rosa26-H3F3B
Plasmid#73131PurposeExpression in Mammalian Cells, Cas9 Nuclease to analize translocationsDepositorInsertspCas9
TagsFlagExpressionMammalianAvailable SinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
fADL-1e-HA-linker-p3
Plasmid#139440PurposeFor cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophageDepositorInsertlinker with pelB-leader, cloning site, HA-tag and p3
ExpressionBacterialAvailable SinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only