Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 28,675 results for: tat

Showing: 11761 - 11780 of 28675 results

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pDMJ2-SARS-CoV-2-Spike (B.1-10c)

Plasmid

#241036

Purpose

Expresses SARS-CoV-2 spike (B.1-10c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-10c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T76R,K113T,K147N,S155R,N439R,T478K,E484V,S494R,K5…

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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MB 39Vm13 ttrSR(m13)-bARGSer

Plasmid

#232472

Purpose

Optimized tetrathionate sensor with acoustic reporter genes (bARGSer)

Depositor

Mikhail Shapiro

Article

Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:

Inserts

ttrS
ttrR
PttrB185-269
bARGSer
AxeTxe

Expression

Bacterial

Mutation

the gene Ser39006_001280 was deleted from the clu…

Promoter

Constitutive (TTGATAGCTAGCTCAGTCCTAGGTATTGTGCTAGC…

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (Gamma)

Plasmid

#241115

Purpose

Expresses SARS-CoV-2 spike (Gamma) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (Gamma) (S SARS-CoV-2)

Expression

Mammalian

Mutation

L18F,T20N,P26S,D138Y,R190S,K417T,E484K,N501Y,D614…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (Delta)

Plasmid

#241113

Purpose

Expresses SARS-CoV-2 spike (Delta) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (Delta) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19R,G142D,E156G,F157del,R158del,L452R,T478K,D614…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (Beta)

Plasmid

#241109

Purpose

Expresses SARS-CoV-2 spike (Beta) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (Beta) (S SARS-CoV-2)

Expression

Mammalian

Mutation

L18F,D80A,D215G,L241del,L242del,A243del,K417N,E48…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-10b)

Plasmid

#241035

Purpose

Expresses SARS-CoV-2 spike (B.1-10b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-10b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K113T,K147N,S155R,Y248D,T478K,E484V,F490R,S494R,K…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-10d)

Plasmid

#241037

Purpose

Expresses SARS-CoV-2 spike (B.1-10d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-10d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69G,Y248D,R403T,N439R,T478K,E484V,F486E,F490R,S4…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-10a)

Plasmid

#241034

Purpose

Expresses SARS-CoV-2 spike (B.1-10a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-10a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69G,K113T,K147N,S155R,T478K,E484V,F490R,S494R,K5…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (Alpha)

Plasmid

#241032

Purpose

Expresses SARS-CoV-2 spike (Alpha) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (Alpha) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69del,V70del,Y144del,N501Y,A570D,D614G,P681H,T71…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-KRAS-G12R-IRES-mCherry

Plasmid

#221023

Purpose

Fluorescent reporter for expressing the KRAS G12R mutant CDS

Depositor

Mohamed Fareh

Article

Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

Insert

KRAS G12R (KRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-KRAS-G12S-IRES-mCherry

Plasmid

#221024

Purpose

Fluorescent reporter for expressing the KRAS G12S mutant CDS

Depositor

Mohamed Fareh

Article

Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

Insert

KRAS G12S (KRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-KRAS-WT-IRES-mCherry

Plasmid

#221019

Purpose

Fluorescent reporter for expressing the KRAS wildtype CDS

Depositor

Mohamed Fareh

Article

Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

Insert

KRAS wildtype (KRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-KRAS-G12A-IRES-mCherry

Plasmid

#221020

Purpose

Fluorescent reporter for expressing the KRAS G12A mutant CDS

Depositor

Mohamed Fareh

Article

Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

Insert

KRAS G12A (KRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-KRAS-G12C-IRES-mCherry

Plasmid

#221021

Purpose

Fluorescent reporter for expressing the KRAS G12C mutant CDS

Depositor

Mohamed Fareh

Article

Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

Insert

KRAS G12S (KRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-KRAS-G12D-IRES-mCherry

Plasmid

#221022

Purpose

Fluorescent reporter for expressing the KRAS G12D mutant CDS

Depositor

Mohamed Fareh

Article

Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

Insert

KRAS G12D (KRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA378 - pBA904 Puro-T2A-GFP CD45 CRISPRa guide 3 (pRCA360 backbone)

Plasmid

#238169

Purpose

Lentiviral CRISPR guide vector expressing PTPRC targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

CD45 sgRNA CRISPRa (PTPRC Human, Synthetic)

Use

CRISPR and Lentiviral

Tags

GFP

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pT/UBC-mVenus-P2A-NRASG12V

Plasmid

#236074

Purpose

Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Ubc promotor driven expression of mVenus and mutant NRAS (NRASG12V).

Depositor

Masashi Narita

Article

Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

Inserts

mVenus
NRAS G12V (NRAS Human)

Use

Sleeping beauty transposon plasmid

Mutation

G12V

Promoter

Ubc

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pT/PGK-mVenus-P2A-NRASG12V

Plasmid

#236073

Purpose

Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Pgk promotor driven expression of mVenus and mutant NRAS (NRASG12V).

Depositor

Masashi Narita

Article

Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

Inserts

mVenus
NRAS G12V (NRAS Human)

Use

Sleeping beauty transposon plasmid

Mutation

G12V

Promoter

PGK

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTK41

Plasmid

#236110

Purpose

Expresses the motor domain of rat KIF5C fused with SNAP tag and ALFA tag nanobody

Depositor

Shinsuke Niwa

Article

Kita et al Cell Rep Phys Sci, 6 (2): 102440. 19 Feb

Inserts

KIF5C(C7S)(1-430)-SNAP-His (Kif5c Rat)
KIF5C(C7S)(1-430)-ALFA tag nanobody-StrepII tag (Kif5c Rat)

Tags

ALFA tag nanobody, His tag, SNAP tag, and StrepII…

Expression

Bacterial

Mutation

C7S mutation is introduced. 1-430 aa is encoded.

Promoter

T7

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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18065-M04-412

Plasmid

#225667

Purpose

Lentivirus protein expression. Control plasmid.

Depositor

Dominic Esposito

Article

Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

Inserts

dsmCherry
delCD19 (CD19 Human)

Use

Lentiviral

Tags

3xFLAG

Mutation

C-terminally truncated (aa 1-333 only)

Promoter

WPRE-SV40p and mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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