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pSLIK-NFLAG-hFUS

Plasmid

#50487

Purpose

Produces lentivirus expressing human FUS with FLAG tag at its N-terminus by co-transfection with psPAX2 and pMD2G

Depositor

Zissimos Mourelatos

Article

Nakaya et al RNA. 2013 Apr;19(4):498-509. doi: 10.126

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

TRE

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only
ECON

Plasmid

#82553

Purpose

Epigenetic effector construct for targeted methylation

Depositor

Joseph Irudayaraj

Article

Choudhury et al Oncotarget. 2016 Jul 4. doi: 10.18632/on

Inserts

DNMT3A (DNMT3A Human)
CRY2 (CRY2 Mustard Weed)

Use

Tags

HALO and mCherry

Expression

Mammalian

Mutation

N-terminal photolyase homology region domain of C…

Promoter

CMV

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-Luc-KLF4-K1S

Plasmid

#62979

Purpose

Translational reporter for KLF4

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 partial sequence (KLF4 Human)

Use

Luciferase

Tags

firefly luciferase

Expression

Mutation

Fragment 1-446 of KLF4

Promoter

CMV

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
SAM-DNMT3A-inactive+sgAAVS1_118

Plasmid

#213171

Purpose

Vector with sgAAVS1 used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Insert

sgAAVS1_118 (AAVS1 Human)

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

E1Fa and U6

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-osr1-KI-donor

Plasmid

#184875

Purpose

Donor template for knock-in of p2a-CreERT2 into the medaka osr1 locus via CRISPR/Cas9-mediated homology directed repair

Depositor

Christoph Winkler

Article

Hui et al Development. 2022 May 20. pii: 275483. d

Inserts

Odd-Skipped Related Transcription Factor 1 5' homology arm
Odd-Skipped Related Transcription Factor 1 3' homology arm
p2a-CreERT2
Myosin light chain 2 promoter
EGFP

Use

CRISPR and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCri-H6-TEV-GRAB

Plasmid

#128498

Purpose

Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)

Use

Tags

His tag (6x)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCri-H6-TEV-GRAB-STREP

Plasmid

#128499

Purpose

Expresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

GRAB (V34-N181) (grab Streptococcus pyogenes serotype M1)

Use

Tags

His tag (6x) and Strep tag (2x)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-Luc-KLF4-K2S

Plasmid

#62980

Purpose

Translational reporter for KLF4

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 partial sequence (KLF4 Human)

Use

Luciferase

Tags

firefly luciferase

Expression

Mutation

Fragment 446-911 of KLF4

Promoter

CMV

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K3S

Plasmid

#62981

Purpose

Translational reporter for KLF4

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 partial sequence (KLF4 Human)

Use

Luciferase

Tags

firefly luciferase

Expression

Mutation

Fragment 911-1406 of KLF4

Promoter

CMV

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K4S

Plasmid

#62982

Purpose

Translational reporter for KLF4

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 partial sequence (KLF4 Human)

Use

Luciferase

Tags

firefly luciferase

Expression

Mutation

Fragment 1406-1781 of KLF4

Promoter

CMV

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K6S

Plasmid

#62983

Purpose

Translational reporter for KLF4

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 partial sequence (KLF4 Human)

Use

Luciferase

Tags

firefly luciferase

Expression

Mutation

Fragment 2086-2639 of KLF4

Promoter

CMV

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pET-His6-ERK2-MEK1_R4F_coexpression

Plasmid

#39212

Depositor

Melanie Cobb

Article

Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

Inserts

Mek1 R4F (MAP2K1 Human)
Erk2 (Mapk1 Rat)

Use

Tags

His6 and RBS

Expression

Bacterial

Mutation

MEK1(R4F) = S218E, S222D and deletion of AA32-51

Promoter

T7 and T7 via RBS

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

Plasmid

#216250

Purpose

HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

HDR template for human SEC61B (SEC61B Synthetic, Human, HDR template)

Use

CRISPR; Hdr template

Tags

BSD.P2A.miniIAA7.3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD S1859A

Plasmid

#169821

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

S1859A; TCCC -> AGTC silent mutations at nt527…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL 5N>Q

Plasmid

#202419

Purpose

Expression of GFP-tagged PODXL with mutated N-linked glycosylation sites

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

N33/43/104/144/360Q

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-ZIP(RK)

Plasmid

#27136

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Arginines in the SH2 domains mutated to lysines (…

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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pHR-ZIP(FF)

Plasmid

#27135

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Tyrosines in ITAMS 1-3 mutated to phenylalanines …

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD S1406A

Plasmid

#169820

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported PKA phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

S1406A; TCCC -> AGTC silent mutations at nt527…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD S1859E

Plasmid

#169822

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

S1859E; TCCC -> AGTC silent mutations at nt527…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-CBP(1603-2678)

Plasmid

#183774

Purpose

Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

Insert

nej (nej Fly)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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