We narrowed to 15,977 results for: LEA

1. pET21b-LaG9(S151C)

   Plasmid

   #172490

   Purpose

   Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

   Depositor

   Siegfried Musser

   Article

   Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

   Insert

   LaG9(S151C)
   

   Tags

   6xHis, PPX cleavage site, and pelB leader sequenc…

   Expression

   Bacterial

   Mutation

   S151C

   Promoter

   T7

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDZ617 pKAN 12xPP7 V4

   Plasmid

   #72237

   Purpose

   Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   12xPP7 V4
   

   Use

   Cre/Lox

   Expression

   Bacterial

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT3TS-nCas9n-A71

   Plasmid

   #125644

   Purpose

   micro-injection of poly-adenylated nCas9n RNA

   Depositor

   Jason Berman

   Article

   Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

   Insert

   nCas9n
   

   Use

   Rna in vitro transcription vector

   Promoter

   T3

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

   Plasmid

   #45578

   Purpose

   Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   coY2 Ani
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDZ645 pKAN 1x-mCherry-12xPP7 V4

   Plasmid

   #73173

   Purpose

   Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   1x-mCherry
   

   Expression

   Mammalian and Yeast

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-(RTRG)6

   Plasmid

   #69849

   Purpose

   The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

   Depositor

   Daniel Christophe

   Article

   Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

   Insert

   6 RTRG repeats
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>

   Plasmid

   #72240

   Purpose

   Histidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   GLT1 GAL1p 24xPP7 V3
   

   Expression

   Bacterial

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. C-term_TEV_aMFx1 (4a)

   Plasmid

   #238903

   Purpose

   Plasmid insert contains the aMFx1 C-terminal tag sequence with the TEV cleavage site for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

   Depositor

   Joseph Brock

   Article

   Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

   Insert

   C-term_TEV_aMFx1
   

   Use

   Synthetic Biology

   Available Since

   Sept. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTrcHisA-NLS-BFP-mEosEM

   Plasmid

   #218934

   Purpose

   Express the nuclear import substrate NLS-BFP-mEosEM.

   Depositor

   Siegfried Musser

   Article

   Sau et al Nature. 2025 Mar 19. doi: 10.1038/s41586

   Insert

   BFP-mEosEM fusion protein
   

   Tags

   6xHis and SV40 nuclear localization sequence (NLS…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2+-Zeo-miR-430

    Plasmid

    #221726

    Purpose

    Expression of Zeocin resistance marker followed by perfect miR-430 target site insertion in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    BleoR-miR-430
    

    Use

    Fish expression

    Promoter

    simian CMV IE94

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEYFP-N1-HsSmg6-mut-siRNAres_I

    Plasmid

    #146588

    Purpose

    Mammalian Expression of HsSmg6-mut-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al RNA. 2008 Dec . 14(12):2609-17.

    Insert

    HsSmg6-mut-siRNAres (SMG6 Human)
    

    Expression

    Mammalian

    Mutation

    two silent mutations T594C, A699G and two non sil…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. KBB416

    Plasmid

    #185087

    Purpose

    Swi6L-GFP with nuclear export signal mutated to alanines

    Depositor

    Ken Belanger

    Article

    Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

    Insert

    SWI6
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Swi6 nuclear export signal L to A mutations

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-CMV-SMCHD1-Exon1-10A.12D-48-3xFLAG

    Plasmid

    #130678

    Purpose

    Expresses human SMCHD1 Exon1-10A.12D-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-10A.12D-48 (SMCHD1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D409_F489del

    Promoter

    CMV

    Available Since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-CMV-SMCHD1-Exon1-36.47M-48-3xFLAG

    Plasmid

    #130680

    Purpose

    Expresses human SMCHD1 Exon1-36.47M-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-36.47M-48 (SMCHD1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D1523_G1960del

    Promoter

    CMV

    Available Since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CMV-SMCHD1-Exon1-46.47A-48-3xFLAG

    Plasmid

    #130677

    Purpose

    Expresses human SMCHD1 Exon1-46.47A-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-46.47A-48 (SMCHD1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    M1961_E1992del

    Promoter

    CMV

    Available Since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CMV-3xHA-SMCHD1-Exon1-14.47M-48-3xFLAG

    Plasmid

    #130691

    Purpose

    Expresses human SMCHD1Exon1-14.47M-48 with an N-terminal 3xHA tag and a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-14.47M-48 (SMCHD1 Human)
    

    Tags

    3xFLAG and 3xHA

    Expression

    Mammalian

    Mutation

    E653_G1960del

    Promoter

    CMV

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CMV-SMCHD1-Exon37-46.47A-48-3xFLAG

    Plasmid

    #130689

    Purpose

    Expresses human SMCHD1 Exon37-46.47A-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon37-46.47A-48 (SMCHD1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    1M_T1522delinsM, M1961_E1992del

    Promoter

    CMV

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CMV-SMCHD1-Exon1-9.47M-48-3xFLAG

    Plasmid

    #130686

    Purpose

    Expresses human SMCHD1 Exon1-9.47M-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-9.47M-48 (SMCHD1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    I378_G1960del

    Promoter

    CMV

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUAS attB V5 dDispatched WT HA

    Plasmid

    #126411

    Purpose

    Expression of wild type dDispatched HA in Drosophila.

    Depositor

    Stacey Ogden

    Article

    Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

    Insert

    d-Dispatched1 (disp Fly)
    

    Tags

    HA

    Expression

    Insect

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-CMV-SMCHD1-Exon37-41S.44R-48-3xFLAG

    Plasmid

    #130690

    Purpose

    Expresses human SMCHD1 Exon37-41S.44R-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon37-41S.44R-48 (SMCHD1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    1M_T1522delinsM, G1720_Y1846del

    Promoter

    CMV

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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