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Showing: 1161 - 1180 of 2836 results

  1. pJET-osr1-KI-donor

    Plasmid
    #184875
    Purpose
    Donor template for knock-in of p2a-CreERT2 into the medaka osr1 locus via CRISPR/Cas9-mediated homology directed repair
    Depositor
    Christoph Winkler
    Article
    Hui et al Development. 2022 May 20. pii: 275483. d
    Inserts
    Odd-Skipped Related Transcription Factor 1 5' homology arm
    Odd-Skipped Related Transcription Factor 1 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    EGFP
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    184875-plasmid-map-sequence-id-366330

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  2. pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-503-WPRE

    Plasmid
    #174133
    Purpose
    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-503 fusion protein under the hSyn promoter
    Depositor
    Florian Freudenberg
    Article
    Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi
    Insert
    Nos1ap (Nos1ap Mouse)
    Use
    AAV
    Tags
    3xFLAG and mCherry
    Expression
    Mutation
    Only contains the sequences coding for amino acid…
    Promoter
    hSyn
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174133-plasmid-map-sequence-id-345689

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  3. pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-427-WPRE

    Plasmid
    #174135
    Purpose
    pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-427 fusion protein under the hSyn promoter
    Depositor
    Florian Freudenberg
    Article
    Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi
    Insert
    Nos1ap (Nos1ap Mouse)
    Use
    AAV
    Tags
    3xFLAG and mCherry
    Expression
    Mutation
    Only contains the sequences coding for amino acid…
    Promoter
    hSyn
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174135-plasmid-map-sequence-id-345687

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  12. pDM304_YFP-IqgC_mRFP-Raf1(RBD)

    Plasmid
    #128698
    Purpose
    simultaneous expression of YFP-tagged IqgC and mRFP-tagged active Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium discoideum cells
    Depositor
    Igor Weber
    Article
    Marinovic et al Proc Natl Acad Sci U S A. 2019 Jan 22;11
    Inserts
    iqgC (full length)
    Raf1 kinase (aa 50-132)
    Use
    Dictyostelium discoideum expression vector
    Tags
    YFP and mRFP
    Expression
    Mutation
    none
    Promoter
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    128698-plasmid-map-sequence-id-246776

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  16. pUDP082

    Plasmid
    #103875
    Purpose
    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103875-plasmid-map-sequence-id-199262

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  18. BPV00500 pET21GG2 His(6)-TF-BECN(1-450)

    Plasmid
    #136684
    Purpose
    Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor)
    Depositor
    Colin Garvie
    Article
    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66
    Insert
    Beclin-1 (BECN1 Human)
    Use
    Tags
    Trigger Factor
    Expression
    Bacterial
    Mutation
    DNA optimized for E. coli expression
    Promoter
    T7
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    136684-plasmid-map-sequence-id-272124

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  20. pUDP025

    Plasmid
    #103874
    Purpose
    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactis
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    HH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103874-plasmid-map-sequence-id-199263

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Showing: 1161 - 1180 of 2836 results