We narrowed to 8,725 results for: Set

1. H14-MBP-SUMO-Cys-Linker-BAF_G47E

   Plasmid

   #101779

   Purpose

   Expresses His-MBP-tagged mutant BAF (G47E) in E.coli (SUMO-cleavable)

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Tags

   His14-MBP-SUMO

   Expression

   Bacterial

   Mutation

   G47E mutation

   Promoter

   T5

   Available Since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-sgRELA

   Plasmid

   #83944

   Purpose

   Lentiviral vector expressing an sgRNA targeting RELA NOTE: This plasmid does NOT contain Cas9.

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   sgRELA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2

   Plasmid

   #186361

   Purpose

   Entry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   Human Ensconsin (MAP7 Human, Synthetic)
   

   Use

   Expression of a fluorescent microtubule marker

   Tags

   3xEGFP

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. H14-MBP-SUMO-FLAG-LEM

   Plasmid

   #101778

   Purpose

   Expresses His-MBP-tagged mutant LEM-domain (FLAG-tagged) in E.coli (SUMO-cleavable)

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   MAN1 (LEMD3 Human)
   

   Tags

   His14-MBP-SUMO-FLAG

   Expression

   Bacterial

   Mutation

   amino acids 6-51 of human MAN1 (LEM domain)

   Promoter

   T5

   Available Since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. F-MED28

   Plasmid

   #15368

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

   Insert

   MED28 (MED28 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pLenti-sgCCR5

   Plasmid

   #83930

   Purpose

   Lentiviral vector expressing an sgRNA targeting CCR5 NOTE: This plasmid does NOT contain Cas9.

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   sgCCR5
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCGT-HCF-1-C600

   Plasmid

   #99364

   Purpose

   C-term of HCFC1 protein

   Depositor

   Winship Herr

   Article

   Wilson et al Mol Cell Biol. 2000 Sep;20(18):6721-30.

   Insert

   HCF-1 C-term 1436-2035 (HCFC1 Human)
   

   Tags

   2x tandem T7 tag and C-myc

   Expression

   Mammalian

   Mutation

   Deletion of HCFC1 cDNA

   Promoter

   CMV

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. H14-MBP-SUMO-Cys-Linker-BAF_L58R

   Plasmid

   #101780

   Purpose

   Expresses His-MBP-tagged mutant BAF (L58R) in E.coli (SUMO-cleavable)

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Tags

   His14-MBP-SUMO

   Expression

   Bacterial

   Mutation

   L58R mutation

   Promoter

   T5

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)GαsS-246-Nluc

   Plasmid

   #207157

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAS (GNAS Human)
   

   Expression

   Mammalian

   Mutation

   NLuc inserted at position D(246)N

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-pSP172BSSPE-R3-ccdB/CmR-R4-bpFOG-B5::B1-NLSLacZ-B2

    Plasmid

    #186414

    Purpose

    AttR3/R4 destination vector for ascidian electroporation with AttB1/B2 recombined NLSLacZ under control of AttB4/B5-recombined pFOG ectodermal regulatory sequence.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    lacZ (lacZ E. coli)
    

    Use

    Gateway destination vector

    Tags

    Nuclear localization signal

    Promoter

    Basal promoter of the FOG gene

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F370G

    Plasmid

    #169960

    Purpose

    Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    FLAG and Ub

    Expression

    Mammalian

    Mutation

    F370G

    Promoter

    CMV

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pInducer20 CARD8-HA PAM L368G

    Plasmid

    #169985

    Purpose

    Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (L368G) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PAM, L368G

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pInducer20 CARD8-HA PAM R394E

    Plasmid

    #169987

    Purpose

    Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (R394E) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PAM, R394E

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pInducer20 CARD8-HA PAM F405G

    Plasmid

    #169988

    Purpose

    Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F405G) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PAM, F405G

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pInducer20 CARD8-HA PAM F370G

    Plasmid

    #169986

    Purpose

    Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F370G) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PAM, F370G

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG L368G

    Plasmid

    #169959

    Purpose

    Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    FLAG and Ub

    Expression

    Mammalian

    Mutation

    L368G

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG R394E

    Plasmid

    #169961

    Purpose

    Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    FLAG and Ub

    Expression

    Mammalian

    Mutation

    R394E

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F405G

    Plasmid

    #170119

    Purpose

    Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed).

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    FLAG and Ub

    Expression

    Mammalian

    Mutation

    F405G

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 LIC 6A CARD8-FLAG E274R + S297A

    Plasmid

    #169951

    Purpose

    Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R) with an additional autoprocessing mutation (S297A)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E274R, S297A

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. RAP2A-p2A-E2Crimson

    Plasmid

    #83940

    Purpose

    Lentiviral vector expressing RAP2A-p2A-E2Crimson

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    p2A-E2Crimson

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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