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Plasmid#198024PurposeLV Expression of CD3D cDNA with mutation at c.275-2A>G used as a disease editing target for CD3d SCID mutation identified in JapanDepositorInsertCD3D c.275-2A>G
UseLentiviralTagsExpressionMammalianMutationCodon optimized (20 bp) on either end of cDNA for…PromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBluescript alpha-SSeCKS
Plasmid#61295Purposecontains rat alpha SSeCKS cDNADepositorInsertalpha SSeCKS (Akap12 Rat)
UseTagsExpressionBacterialMutationcDNA with 1kb of 3' UTR and 150bp of 5'…PromoterT7Available sinceDec. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-ECFP
Plasmid#48353PurposeContributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertECFP
UseGateway entry vectorTagsExpressionMutationContains a stop codon.PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-EYFP
Plasmid#48351PurposeContributes the coding sequence for EYFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertEYFP
UseGateway entry vectorTagsExpressionMutationContains a stop codon.PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-ECFP
Plasmid#48352PurposeContributes the coding sequence for ECFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertECFP
UseGateway entry vectorTagsExpressionMutationContains a Kozak sequence. Does not contain a sto…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-SV40 polyA signal
Plasmid#48627PurposeContributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.DepositorInsertSV40 early polyadenylation signal cassette
UseGateway entry vectorTagsExpressionMutationPromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-EYFP
Plasmid#48350PurposeContributes the coding sequence for EYFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertEYFP
UseGateway entry vectorTagsExpressionMutationContains a Kozak sequence. Does not contain a sto…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-mKate2
Plasmid#48345PurposeContributes the coding sequence for the mKate2 fluorescent protein as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertmKate2
UseGateway entry vectorTagsExpressionMutationContains a stop codon at the 3' end of the c…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-mKate2
Plasmid#48344PurposeContributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertmKate2
UseGateway entry vectorTagsExpressionMutationIt contains a Kozak sequence but no stop codon.PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.MCS.IRES.GFP
Plasmid#169280PurposeConstitutive overexpression of cDNA with GFP as a reporter fluorescent proteinDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.MCS.IRES.dTomato
Plasmid#169279PurposeConstitutive overexpression of cDNA with dTomato as a reporter fluorescent proteinDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-EGFP
Plasmid#48349PurposeContributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertEGFP
UseGateway entry vectorTagsExpressionMutationAminoacid 40 is valine in our sequence, while it …PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-V5-6xHis
Plasmid#48347PurposeContributes the coding sequence of a V5-6xHis epitope tags cassette as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertV5 and 6xHis epitope tags cassette
UseGateway entry cloneTagsExpressionMutationContains a stop codon at the end of the epitope t…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-EGFP
Plasmid#48348PurposeContributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertEGFP
UseGateway entry vectorTagsExpressionMutationContains a Kozak sequence. Does not contain a sto…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-V5-6xHis
Plasmid#48346PurposeContributes the coding sequence of a V5-6xHis epitope tags cassette as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertV5 and 6xHis epitope tags cassette
UseGateway entry cloneTagsExpressionMutationContains a Kozak sequence and initiation codon up…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.TRE.MCS.IRES.dTomato.PGK.sfGFP.P2A.Tet3G
Plasmid#169283PurposeDoxycycline-inducible overexpression of cDNA with dTomato as a reporter fluorescent proteinDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV WS myc
Plasmid#19274DepositorInsertmouse Wrn cDNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBlue-hFX(I154F)(AA88)-HA
Plasmid#15403DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1, HA, and HISExpressionBacterialMutationcontains cDNA coding sequence for amino acids 88-…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28(a)-hFX(I154F)-HA
Plasmid#14992DepositorInserthuman frataxin cDNA (FXN Human)
UseTagsAU1, HA, and HISExpressionBacterialMutationcontains cDNA coding sequence for amino acids 88-…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
mouse Capping Protein Alpha2 EGFP
Plasmid#229805PurposeMamalian expressionDepositorInsertmouse capping protein Alpha2 EGFP fusion cDNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only