We narrowed to 5,570 results for: donor

1. AAVS1-TetOn-3XFLAG-firefly-beta-globin-control-AAVS1

   Plasmid

   #184395

   Purpose

   Donor plasmid for stable integration of firefly luciferase control reporter at AAVS1

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Firefly luciferase beta-globin
   

   Use

   CRISPR; Donor plasmid for hdr

   Tags

   3X FLAG

   Expression

   Mammalian

   Promoter

   Tet-On 3G

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMIA4.721-Keppel

   Plasmid

   #214444

   Purpose

   BxbI landing-pad HDR donor for targeting Keppel-19 GSH. CAGG-driven Casp9-P2A-Bsd.

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   2xFKBP12-Casp9
   

   Use

   Hdr donor

   Tags

   P2A-Bsd

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMIA4.721-Olônne

   Plasmid

   #214443

   Purpose

   BxbI landing-pad HDR donor for targeting Olônne-18 GSH. CAGG-driven Casp9-P2A-Bsd.

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   2xFKBP12-Casp9
   

   Use

   Hdr donor

   Tags

   P2A-Bsd

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-AAVS1-PGK-Puro-moxGFP

   Plasmid

   #227275

   Purpose

   AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

   Plasmid

   #45564

   Purpose

   Expresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequence

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   d14 eGFP donor
   I-Sce I Nuclease
   BFP
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted n' 15 amino acids

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAVS1-TetOn-3XFLAG-renilla-beta-globin-control-AAVS1

   Plasmid

   #184396

   Purpose

   Donor plasmid for stable integration of Renilla luciferase control reporter at AAVS1

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Renilla luciferase beta-globin
   

   Use

   CRISPR; Donor plasmid for hdr

   Tags

   3X FLAG

   Expression

   Mammalian

   Promoter

   Tet-On 3G

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOD_C2906

   Plasmid

   #91093

   Purpose

   Module C, Promoter: FMV 34S, Gene: TREX2 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TREX2 (+ BaeI site for GT donor cloning) (Trex2 Mouse)
   

   Use

   Unspecified

   Promoter

   FMV 34S

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-Solo-mStayGold-PXN

   Plasmid

   #227316

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   PXN Homology Arms flanking a mStayGold Tag (PXN Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-Solo-mScarlet-H2BC11

   Plasmid

   #207756

   Purpose

   Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pAC84-pCR8-dCas9

    Plasmid

    #48218

    Purpose

    dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A)
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag

    Mutation

    D10A;H840A

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223 PAK5

    Plasmid

    #60532

    Purpose

    Donor vector for human Pak5

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    Pak5 (PAK5 Human)
    

    Use

    Gateway donor vector

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pJG367

    Plasmid

    #91185

    Purpose

    BeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    nAtCas9_H840A+gNt_R2+gNt_F2+donor
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJG382

    Plasmid

    #91189

    Purpose

    BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    nAtCas9_D10A+gNt_R2+gNt_F2+donor
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221-rap1-K9R

    Plasmid

    #84527

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Mutation

    K(43,61,69,117,240,246,527,582,651)R

    Promoter

    none

    Available Since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pJG488

    Plasmid

    #91196

    Purpose

    WDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    nTaCas9_H840A+gUbi8+gUbi1+donor
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221-rap1-K462-463-651R

    Plasmid

    #84564

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Mutation

    K462-463-651R

    Promoter

    none

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221-rap1-K43-117-118R

    Plasmid

    #84565

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Mutation

    K43-117-118R

    Promoter

    none

    Available Since

    Oct. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGTPA

    Plasmid

    #171525

    Purpose

    Plasmid contains the Cas12k gRNA and transposon donor cassette

    Depositor

    Quanjiang Ji

    Article

    Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:

    Insert

    Cas12k gRNA, transposon donor cassette
    

    Use

    CRISPR

    Promoter

    Constitutive promoter J23119

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSCL475

    Plasmid

    #219503

    Purpose

    Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

    Insert

    Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
    

    Expression

    Yeast

    Promoter

    GAL7

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28b-MBP-bhaB5

    Plasmid

    #167812

    Purpose

    Expresses His6- and MBP-tagged BhaB5 in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

    Insert

    BhaB5
    

    Tags

    His6, MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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