We narrowed to 2,567 results for: gem

1. pMMK ENTR 4

   Plasmid

   #206263

   Purpose

   ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Type

   Empty backbone

   Use

   Multimate/gateway entr 4

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFBD-Akt1(WT)

   Plasmid

   #86561

   Purpose

   Expresses human full-length Akt1(WT)

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Tags

   10xHis

   Expression

   Insect

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVPS13D-I-EGFP

   Plasmid

   #176464

   Purpose

   express internally GFP-tagged VPS13D in mammalian cells

   Depositor

   Richard Youle

   Article

   Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   VPS13D (VPS13D Human)
   

   Tags

   GFP-Ub

   Expression

   Mammalian

   Mutation

   EYFP is internally inserted

   Promoter

   CMV

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV Syn ChR E90R-T159C 2A tDimer

   Plasmid

   #52495

   Purpose

   Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

   Depositor

   Thomas Oertner

   Article

   Wietek et al Science. 2014 Mar 27.

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   E90R, T159C

   Promoter

   human synapsin-1 and ribosomal skip sequence

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-X1-Neo-NDUFB2

   Plasmid

   #139841

   Purpose

   Lentiviral expression of human NDUFB2

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   NDUFB2 (NDUFB2 Human)
   

   Use

   Lentiviral

   Available Since

   July 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFKBPmCherry-Akt2(K14A)

   Plasmid

   #86597

   Purpose

   Expresses recruitable mCherry-tagged human Akt2(K14A) membrane-binding mutant

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   FKBB-mCherry-Akt2(K14A) (AKT2 )
   

   Expression

   Mammalian

   Available Since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-Akt2(K14A/R25E)

   Plasmid

   #86595

   Purpose

   Expresses eGFP-tagged human Akt2(K14A/R25E) membrane-binding mutant

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt2(K14A/R25E) (AKT2 )
   

   Expression

   Mammalian

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCer-Vamp2(qv/fw)

   Plasmid

   #53238

   Purpose

   Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   VAMP2 (Vamp2 Rat)
   

   Tags

   4aa-linker and mCerulean

   Expression

   Mammalian

   Mutation

   Mutated Q76 to V; F77 to W.

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pJRTCLCrVA.008

   Plasmid

   #22841

   Depositor

   Niki Robertson

   Article

   Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

   Insert

   A DNA (with MCS)
   

   Use

   Cotton vigs vector

   Expression

   Bacterial

   Mutation

   Coat Protein (AR1 gene) of Cotton Leaf Crumple Vi…

   Available Since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-Akt2(T309A)

    Plasmid

    #86625

    Purpose

    Expresses eGFP-tagged human Akt2(T309A) phosphomutant

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt2(T309A) (AKT2 Human)
    

    Expression

    Mammalian

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS

    Plasmid

    #63170

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-OLLAS"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJRTCLCrVB1.3

    Plasmid

    #22844

    Depositor

    Niki Robertson

    Article

    Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

    Insert

    B DNA
    

    Use

    Cotton vigs vector

    Expression

    Bacterial

    Mutation

    1.3 unit B componet length inserted into pBlueScr…

    Available Since

    March 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-sgKmt2d-sg3

    Plasmid

    #192167

    Purpose

    Lenti-sgKmt2d-sg3

    Depositor

    Sidi Chen

    Article

    Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

    Type

    Empty backbone

    Use

    Lentiviral

    Mutation

    NA

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTH33 [pmyo-3::YFP-BeCyclOp(A-2x)::SL2::mCherry]

    Plasmid

    #168169

    Purpose

    Expression of YFP-BeCyclOp(A-2x) in BWMs of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

    Insert

    YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)
    

    Tags

    YFP

    Expression

    Worm

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGFP-Ub-VPS13D_dC

    Plasmid

    #176466

    Purpose

    express VPS13D lacking C-terminal 407 aa in mammalian cells

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    C-terminal 407 aa is deleted.

    Promoter

    CMV

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pESUMO-hRORg2

    Plasmid

    #170329

    Purpose

    Expresses RORg isoform 2 with N-terminal HisSUMO tag in bacteria

    Depositor

    Patrick Griffin

    Article

    Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

    Insert

    RORG isoform 2 (RORC Human)
    

    Tags

    HisSUMO

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    T7

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJBL3664

    Plasmid

    #162240

    Purpose

    Transcribes E. coli SRP RNA sequence for SHAPE-Seq

    Depositor

    Julius Lucks

    Article

    Yu et al Mol Cell. 2021 Feb 18;81(4):870-883.e10.

    Inserts

    E. coli SRP RNA
    HDV
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCherry-Akt2(K14A)

    Plasmid

    #86624

    Purpose

    Expresses mCherry-tagged human Akt2(K14A) membrane binding mutant

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt2(K14A) (AKT2 )
    

    Expression

    Mammalian

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-X1-Neo-NDUFB4

    Plasmid

    #139840

    Purpose

    Lentiviral expression of human NDUFB4

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    NDUFB4 (NDUFB4 Human)
    

    Use

    Lentiviral

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV CaMKIIa ChR2 E90R D156N T159C 2A tDimer

    Plasmid

    #52878

    Purpose

    Chloride-conducting Channelrhodopsin with slow kinetics, neuron-specific promoter, plus red fluorescent protein, excitatory neurons

    Depositor

    Thomas Oertner

    Article

    Wietek et al Science. 2014 Mar 27.

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E90R, D156N, T159C

    Promoter

    CaMKIIa

    Available Since

    July 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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