We narrowed to 25,057 results for: Nes

1. GFP-mROCK1-delta5

   Plasmid

   #101293

   Purpose

   Mammalian expression of Rock1-GFP for the study of localisation signaling.

   Depositor

   Alpha Yap

   Article

   Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

   Insert

   Rock1 (Rock1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   375-727 aa

   Promoter

   CMV

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMOD_A0301

   Plasmid

   #91005

   Purpose

   Module A, Promoter: 35S, Gene: AtCas9_H840A nickase, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9_H840A nickase
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   H840A

   Promoter

   35S

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCerulean3-PTK2-N-10

   Plasmid

   #55442

   Purpose

   Localization: FAK/Focal Adhesions, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   PTK2 (PTK2 Human)
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. Tol2-lyzC-microRNA 722-Dendra2

   Plasmid

   #97130

   Purpose

   overexpression of microRNA and Dendra2 in Zebrafish

   Depositor

   Qing Deng

   Article

   Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

   Insert

   microRNA 722 Dendra2 (mir722 Zebrafish)
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. XE28 XBC 40

   Plasmid

   #16842

   Depositor

   Randall Moon

   Article

   Yost et al Genes Dev. 1996 Jun 15. 10(12):1443-54.

   Insert

   beta-catenin-myc (ctnnb1.L Frog)
   

   Use

   Xenopus expression

   Tags

   6x myc

   Expression

   Mutation

   Promoter

   Available Since

   May 22, 2009

   Availability

   Academic Institutions and Nonprofits only

6. p5E-pax3a-5.4k

   Plasmid

   #164966

   Purpose

   zebrafish pax3a -5.4k promoter

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   pax3a (pax3a Zebrafish)
   

   Use

   Gateway 5 end entry vector

   Tags

   Expression

   Mutation

   promoter -5.4k

   Promoter

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBSHSP:FLP;CMV:tdTomato-SceI

   Plasmid

   #30523

   Depositor

   Gerhart Ryffel

   Article

   Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

   Insert

   FLP recombinase tdTomato
   

   Use

   Flp/frt, heat shock inducible, i-scei megabase ve…

   Tags

   Expression

   Mutation

   R415H

   Promoter

   Available Since

   Oct. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. Desmocollin2_tail-GST

   Plasmid

   #36992

   Depositor

   Kathleen Green

   Article

   Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

   Insert

   Dsc2 (DSC2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Intracellular domain of Dsc2 (nt 2,603–3,158)

   Promoter

   Available Since

   July 31, 2012

   Availability

   Academic Institutions and Nonprofits only

9. BII-ChBtW-PLAGL2

   Plasmid

   #133369

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   PLAGL2 (PLAGL2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. MS2_adRNA

    Plasmid

    #124624

    Purpose

    Expresses MS2_adRNA targeting the RAB7A transcript along with GFP

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    MS2_adRNA_RAB7A
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. px330 EQR

    Plasmid

    #101733

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

    Promoter

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pG3K-U6EC1

    Plasmid

    #134757

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    gRNA-U6, zCas9-EC1

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pG3B-U6EC1

    Plasmid

    #134756

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    gRNA-U6, zCas9-EC1

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pG3H-U3Ub

    Plasmid

    #134749

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    gRNA- OsU3, zCas9-Ubi1

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. RNAPII_CTD38-52

    Plasmid

    #98685

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    heptads 38-52, residues 1853-1970

    Promoter

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRUSH

    Plasmid

    #54479

    Purpose

    Mouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to drive short RNAs, pgkneo, cassette flanked by loxP sites

    Depositor

    Laurie Jackson-Grusby

    Article

    Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1

    Type

    Empty backbone

    Use

    Cre/Lox, Mouse Targeting, and RNAi

    Tags

    SA-EGFP-polyA, U6 promoter, loxP, and pgk-neo

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCMV-Flag-YAP-S384A

    Plasmid

    #33070

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S384A

    Promoter

    CMV

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1(+) EPHB4 Antisense

    Plasmid

    #60640

    Purpose

    Negative Control for the pcDNA3.1(+) EPHB4 Sense plasmid

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    Ephrin type B receptor 4 (EPHB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCFJ2214 - Intron GG1 - 900 bp

    Plasmid

    #159880

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG1 - 900 bp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMOD_A3910

    Plasmid

    #91054

    Purpose

    Module A, Promoter: ZmUbi, Gene: dTaCas9-VPR, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    dTaCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ZmUbi

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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