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pMC-XI2

Plasmid

#176156

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pJOG417

Plasmid

#105341

Purpose

Yeast two hybrid

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG418

Plasmid

#105342

Purpose

Yeast two hybrid

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMEXC3GH (Kan)

Plasmid

#110081

Purpose

Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMINTC3GH (Kan)

Plasmid

#110076

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA KanR neo

Plasmid

#167909

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJRA99

Plasmid

#209334

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of untagged protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMAD-Gate2

Plasmid

#25941

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

Insert

Tags

VP16 Fusion Protein

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pRS423-TyrSgH

Plasmid

#163973

Purpose

Helper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoter

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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plIVMD156

Plasmid

#160934

Purpose

In vivo mRNA display Gateway Destination construct

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop

Tags

HIS tag, 3'UTR MS2 Stem Loop

Expression

Yeast

Promoter

MET25

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pM115: CasRx control presgRNA

Plasmid

#166867

Purpose

U6-driven expression of control (non-targeting) presgRNA compatible with CasRx.

Depositor

Guei-Sheung Liu

Article

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Insert

non-targeting presgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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p005kanRFP

Plasmid

#58533

Purpose

RFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

Red fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

T5 between two lac operators

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pME-TagRFPt-NS

Plasmid

#75343

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagRFPt

Depositor

Rob Parton

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

TagRFPt

Expression

Mammalian

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pICSL50015

Plasmid

#50318

Purpose

Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, mNEON (Branchistoma lanceolatum)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pJRA-GW

Plasmid

#209333

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-X4

Plasmid

#176154

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-4

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-XII1

Plasmid

#176159

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-1

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XI3

Plasmid

#176157

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-3

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XII5

Plasmid

#176161

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-5

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pME-nlsGal80(opt)

Plasmid

#51618

Purpose

vertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stop

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

Gal80(opt) (GAL80 Budding Yeast)

Use

Vertebrate expression

Tags

NLS

Mutation

codon optimized

Available Since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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