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CROP_C9_Puro_AR

Plasmid

#183271

Purpose

All-in-One CRISPRko system with a guide RNA that targets AR gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AR

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_ALK

Plasmid

#183270

Purpose

All-in-One CRISPRko system with a guide RNA that targets ALK gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ALK

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_ADA

Plasmid

#183269

Purpose

All-in-One CRISPRko system with a guide RNA that targets ADA gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ADA

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pPRIChp-hOtub1

Plasmid

#174743

Purpose

Gene overexpression

Depositor

Shao-Cong Sun

Article

Zhou et al Nat Immunol. 2019 Jul;20(7):879-889. doi

Insert

OTUB1 (OTUB1 Human)

Use

Retroviral

Tags

HA

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-E309C-W340A-CAAX.pcDNA3-k

Plasmid

#127409

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon, E309C …

Promoter

CMV

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A-CAAX.pcDNA3-k

Plasmid

#127399

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon and W34…

Promoter

CMV

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-E309C-W340A.pcDNA3-k

Plasmid

#127408

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon, E309C …

Promoter

CMV

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-K305H-E309H-W340A.pcDNA3-k

Plasmid

#127406

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon, K305H,…

Promoter

CMV

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-MBP1-E322TAG-W340A-CAAX.pcDNA3-k

Plasmid

#127405

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

CAAX and FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon and W34…

Promoter

CMV

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-K295TAG-W340A-S233H-T237C.pcDNA3-k

Plasmid

#127402

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon, W340A,…

Promoter

CMV

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-MBP1-K295TAG-W340A-S233H-T237H.pcDNA3-k

Plasmid

#127400

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: K295 to Amber codon, W340A,…

Promoter

CMV

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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Ubiquitin K6C/G76C

Plasmid

#86584

Purpose

Bacterial expression of Ubiquitin K6C/G76C

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; K6C/G76C

Promoter

Tac

Available Since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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Ubiquitin K48C/G76C

Plasmid

#86585

Purpose

Bacterial expression of Ubiquitin K48C/G76C

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; K48C/G76C

Promoter

Tac

Available Since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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Ubiquitin K63C/G76C

Plasmid

#86586

Purpose

Bacterial expression of Ubiquitin K63C/G76C

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; K63C/G76C

Promoter

Tac

Available Since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-KG/Net1A

Plasmid

#28261

Depositor

Jeffrey Frost

Article

Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

Insert

Net1A (Net1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

April 11, 2011

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 EPSM

Plasmid

#27222

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A

Available Since

Dec. 21, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 AAGY

Plasmid

#27013

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

P181A P182A

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pGEX cycT2b (1-589) (P#1732)

Plasmid

#14604

Depositor

Matija Peterlin

Article

Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

Insert

cycT2 (1-589) (CCNT2 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-589

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode

Plasmid

#170255

Purpose

Barcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

SV40 Large T Antigen

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C9orf72

Plasmid

#83437

Purpose

Expresses GFP-C9orf72 in mammalian cells

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

C9orf72 (C9orf72 Human)

Tags

GFP

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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