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pGEX-6p-2-GST-PD-1icd (AA194-288, Y223F, Y248F)

Plasmid

#180815

Purpose

Expression of GST fused human PD-1 intracellular domain (AA194-288, Y223F, Y248F)

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

PD-1int (AA194-288, Y223F, Y248F) (PDCD1 Human)

Expression

Bacterial

Mutation

PD-1 (Y223F, Y248F)

Promoter

Tac

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Gpi1

Plasmid

#175934

Purpose

knockout mouse Gpi1

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Gpi1 gRNA (Gpi1 Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo E55A E57A K58A nsp1

Plasmid

#175427

Purpose

Expresses E55A E57A K58A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nsp1 E55A E57A K58A

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

N-terminal mutants E55A E57A K58A

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2(delta)KL-VSV

Plasmid

#139352

Purpose

Expression of TYK2 protein missing the kinase-like (pseudokinase) domain

Depositor

Sandra Pellegrini

Article

Velazquez et al J Biol Chem. 1995 Feb 17;270(7):3327-34.

Insert

TYK2 cDNA deleted of aa594-877 with a Tyr inserted after aa593 (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F-deletion of aa 594-877

Promoter

CMV

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2(delta)TK-VSV

Plasmid

#139353

Purpose

Expression of TYK2 protein missing the tyrosine kinase (TK) domain (aa 1-895)

Depositor

Sandra Pellegrini

Article

Velazquez et al J Biol Chem. 1995 Feb 17;270(7):3327-34.

Insert

TYK2 cDNA expressing aa 1-895 (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F-deletion of aa 896-1187

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTH2-L22V

Plasmid

#116639

Purpose

Lentiviral expression of PTH2 L22V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTH2 (PTH2 Human)

Use

Lentiviral

Mutation

L22V

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTH2

Plasmid

#116781

Purpose

Lentiviral expression of PTH2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTH2 (PTH2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-NOTCH2NL

Plasmid

#116766

Purpose

Lentiviral expression of NOTCH2NL

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

NOTCH2NL (NOTCH2NLA Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-TBC1D3B-R162Q

Plasmid

#116694

Purpose

Lentiviral expression of TBC1D3B R162Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

TBC1D3B (TBC1D3B Human)

Use

Lentiviral

Mutation

R162Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-WDR12-Q93K

Plasmid

#116706

Purpose

Lentiviral expression of WDR12 Q93K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

WDR12 (WDR12 Human)

Use

Lentiviral

Mutation

Q93K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-TBC1D3B-G164E

Plasmid

#116692

Purpose

Lentiviral expression of TBC1D3B G164E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

TBC1D3B (TBC1D3B Human)

Use

Lentiviral

Mutation

G164E

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-NOTCH2NL-G137L

Plasmid

#116439

Purpose

Lentiviral expression of NOTCH2NL G137L

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

NOTCH2NL (NOTCH2NLA Human)

Use

Lentiviral

Mutation

G137L

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-NOTCH2NL-Q172_G173delinsHC

Plasmid

#116440

Purpose

Lentiviral expression of NOTCH2NL Q172_G173delinsHC

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

NOTCH2NL (NOTCH2NLA Human)

Use

Lentiviral

Mutation

Q172_G173delinsHC

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-NOTCH2NL-R87M

Plasmid

#116441

Purpose

Lentiviral expression of NOTCH2NL R87M

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

NOTCH2NL (NOTCH2NLA Human)

Use

Lentiviral

Mutation

R87M

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-NOTCH2NL-S165F

Plasmid

#116442

Purpose

Lentiviral expression of NOTCH2NL S165F

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

NOTCH2NL (NOTCH2NLA Human)

Use

Lentiviral

Mutation

S165F

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-OXA1L-L57F

Plasmid

#116445

Purpose

Lentiviral expression of OXA1L L57F

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

OXA1L (OXA1L Human)

Use

Lentiviral

Mutation

L57F

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-OXA1L-P58S

Plasmid

#116446

Purpose

Lentiviral expression of OXA1L P58S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

OXA1L (OXA1L Human)

Use

Lentiviral

Mutation

P58S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRA/PINCO

Plasmid

#108938

Purpose

Retroviral expression of mRIN2 dRA

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Deletion of RA domain (aa 751-836)

Promoter

LTR

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRHVPS9/PINCO

Plasmid

#108939

Purpose

Retroviral expression of mRIN2 dRHVPS9

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Deletion of RHVPS9 domain (aa 455-738)

Promoter

LTR

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_ZNF597_p.R247T

Plasmid

#82919

Purpose

Gateway Donor vector containing ZNF597, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZNF597 (ZNF597 Human)

Use

Gateway Cloning

Mutation

R247T

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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